Presence and importance of oxidative stress parameters in malignant mammary gland tumors in dogs

• Published in: Veteriner fakultesi dergisi Vol. 27; no. 4; pp. 517 - 523
• Main Authors: Emin KARAKURT, Mushap KURU, Serpil DAĞ, Enver BEYTUT, Hasan ORAL, Hilmi NUHOĞLU, Ayfer YILDIZ
• Format: Journal Article
• Language: English
• Published: Kafkas University, Faculty of Veterinary Medicine 01.07.2021
• Subjects: canine; carcinoma; mammary gland; oxidative stress
• ISSN: 1309-2251
• DOI: 10.9775/kvfd.2021.25919

This study aimed to evaluate the presence of oxidative stress based on lipid peroxidation and the DNA damage markers malondialdehyde (MDA) and 8-hydroxy-2"-deoxyguanosine (8-OHdG) in canine mammary gland carcinomas using immunohistochemistry techniques. A total of ten malignant and six normal canine mammary tissue samples were evaluated. The specimens were fixed in 10% buff ered formaldehyde solution, processed routinely, embedded in paraffin wax, sectioned at 5 μm, stained with hematoxylin and eosin, examined under a light microscope, and photographed to detect histopathological changes. For immunohistochemistry, the avidin-biotin-peroxidase method was performed. All canine mammary gland tumors were immunopositive for MDA and 8-OHdG expression. There was a statistically significant increase in MDA and 8-OHdG expressions in the tumor group compared to the control group. Based on this study data, in the context of oxidative stress, it is proposed that lipid peroxidation and reactive oxygen species (ROS)-induced DNA damage are significantly associated with canine mammary gland tumor development. In addition, antioxidants may be useful in the treatment of canine mammary gland tumors.