Do snails Lymnaea stagnalis have phenoloxidase activity in hemolymph?

Hemocytes and hemolymph of snail Lymnaea stagnalis were analyzed to detect phenoloxidase (PO) activity. No PO activity was found in the hemocytes of snails. A low level of PO activity (by DOPA oxidation) was registered in the hemolymph without cells. Addition of a specific PO inhibitor revealed the...

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Bibliographic Details
Published inInvertebrate survival journal Vol. 12; pp. 5 - 12
Main Authors YL Vorontsova, IA Slepneva, NI Yurlova, VV Glupov
Format Journal Article
LanguageEnglish
Published University of Modena and Reggio Emilia 01.01.2015
Subjects
phenoloxidase; peroxidase; Lymnaea stagnalis; immunity
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Summary:Hemocytes and hemolymph of snail Lymnaea stagnalis were analyzed to detect phenoloxidase (PO) activity. No PO activity was found in the hemocytes of snails. A low level of PO activity (by DOPA oxidation) was registered in the hemolymph without cells. Addition of a specific PO inhibitor revealed the lack of effect on enzyme activity in the hemolymph, whereas hydrogen peroxide has increased it. Studying the electron paramagnetic resonance (EPR) spectrum of DOPA- and dopamine-semiquinone indicates the peroxide-dependent dopamine oxidation. Our results suggest that peroxidase, rather than phenoloxidase, plays a key role in the oxidation of DOPA and dopamine in the hemolymph of L. stagnalis. It is just peroxidase activity that may be important in the formation of cytotoxic molecules, such as o-semiquinones, during snail defense immune reactions
ISSN:1824-307X