EFFECT OF GAMMA RAY STERILISATION ON DIFFERENTLY DECELLULARISED OMENTUM BASED SCAFFOLDS

Terminal sterilisation of extracellular matrix scaffolds is an essential step in biomedical applications as well as ensuring product safety. Selection of appropriate sterilisation method is crucial in order to retain the structural and mechanical properties of the scaffold. The present study evaluat...

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Published inExploratory animal and medical research Vol. 9; no. 2; pp. 174 - 179
Main Authors Ashna S., Dhanush Krishna B, Sajitha I.S, Vasudevan V.N, Pavan M, Mammen J. Abraham
Format Journal Article
LanguageEnglish
Published West Bengal Veterinary Alumni Association 01.12.2019
Subjects
bile
extracellular matrix
gamma ray irradiation
omental scaffold
texture profile analyser
triton x-100
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Summary:Terminal sterilisation of extracellular matrix scaffolds is an essential step in biomedical applications as well as ensuring product safety. Selection of appropriate sterilisation method is crucial in order to retain the structural and mechanical properties of the scaffold. The present study evaluated the effect of gamma ray sterilisation (25KGy) on biomechanical properties of differently decellularised omental scaffolds. Triton X- 100 and bile were used as decellularising agents and un-processed defattened omentum as control. Effect of irradiation on maximum force, tensile strength and Young’s modulus before and after irradiation was evaluated using Texture Profile Analyser. Our results indicated that, gamma ray irradiation reduced the maximum force and tensile strength of differently processed omental scaffolds. Bile treatment was found to have more protective action on the structural component of the scaffolds than triton X-100. Thus, bile treated omental scaffold showed better biomechanical properties for maximum force and tensile strength than triton X- 100 after irradiation.
ISSN:2277-470X
2319-247X