Development and validation of lc-ms/ms method for determination of ten beta agonists in bovine urine

• Published in: Veteriner fakultesi dergisi Vol. 25; no. 1; pp. 55 - 60
• Main Authors: Risto UZUNOV, Zehra HAJRULAI-MUSLIU, Velimir STOJKOVSKI, Elizabeta DIMITRIESKA-STOJKOVIC, Biljana STOJANOVSKA-DIMZOSKA, Pavle SEKULOVSKI, Dean JANKULOSKI
• Format: Journal Article
• Language: English
• Published: Kafkas University, Faculty of Veterinary Medicine 01.01.2019
• Subjects: bovine; lc-ms/ms; urine; validation; β-agonists
• ISSN: 1309-2251
• DOI: 10.9775/kvfd.2018.20324

The use of β-agonists in livestock production is prohibited in many countries because the residues of β-agonists in food pose a potential risk to human health. The present work describes the development and validation of the LC-MS/MS method for detection of ten β-agonists in bovine urine according to Commission Decision 2002/657/EC requirements. Linearity of the method resulted with coefficient of correlation >0.990. The decision limits (CCα) ranged from 0.127 ng/mL to 0.646 ng/mL, and the detection capability (CCβ) resulted in the range 0.140 ng/mL to 0.739 ng/mL. The observed recoveries in the fortified bovine urine samples were satisfactory at every fortification level with values from 73.67% to 118.80%. The coefficient of variation (CV, %) at three fortification levels for each β-agonist was in complete agreement with the requirements from Commission Decision 2002/657/EC. The CV for intraday precision varied from 1.619% to 15.472% and the CV for interday precision varied from 2.695% to 10.441%. From the obtained validation results the proposed method is an appropriate method for determination of β-agonists in bovine urine.