Influence of MSA on cell growth and spontaneousn metastasis of L9981-Luc lung cancer transplanted model in nude mice by bioluminescence imaging

• Published in: Zhongguo fei ai za zhi Vol. 16; no. 2; pp. 67 - 72
• Main Authors: Ren, Yuanrong, Wang, Yuli, Liu, Hongyu, Yan, Huiqin, Chen, Jun, Hou, Mei, Li, Weimin, Fan, Yaguang, Zhou, Qinghua
• Format: Journal Article
• Language: Chinese
• Published: China Chinese Anti-Cancer Association Chinese Antituberculosis Association 01.02.2013; Chinese Anti-Cancer Association; Chinese Antituberculosis Association
• Subjects: Acids; Animals; Antineoplastic Agents - pharmacology; Apoptosis; Bioluminescence; Bioluminescence imaging; Body Weight - drug effects; Cell Line, Tumor; Cell Proliferation - drug effects; Humans; Luminescent Measurements; Lung cancer; Lung neoplasms; Lung Neoplasms - pathology; Metastasis; Methylseleninic acid; Mice; Mice, Nude; Molecular Imaging; Neoplasm Metastasis; Organoselenium Compounds - pharmacology; Time Factors; Xenograft Model Antitumor Assays; Sacramento California; United States > US; China
• ISSN: 1009-3419; 1999-6187
• DOI: 10.3779/j.issn.1009-3419.2013.02.02

Methylseleninic acid (MSA) is an artificially developed selenium compound. It has been proven that MSA could inhibit growth and metastasis on many tumor cells. This study investigated whether MSA has an impact on the growth and metastasis of L9981-Luc lung cancer transplanted model in nude mice or not. A transplantated tumor model was established in nude mice. Fifteen nude mice were randomly divided into three groups: the control group treated with normal saline (0.2 mL/d), the MSA group treated with MSA solution (0.2 mL), and the cisplatin (DDP) group injected intraperitoneally with DDP (4 mg/kg/w). Inhibition of MSA on tumor growth and tumor metastasis was observed using the IVIS Imaging System 200 Series. A significant difference was obserced in the primary tumor bioluminescence among the three groups (P=0.002) on 21 days post-inoculation. Primary tumor bioluminescence in the DDP group (P=0.001) and in the MSA group (P=0.031) was significantly lower than that in the control group (P=0.001). No significant difference in the metastasis bioluminescence of the thoracic area was indicated among the three groups (P>0.05). MSA can inhibit the growth of planted tumor of transgenic lung cancer cell lines L9981-Luc in nude mice. MSA may also suppress the distant metastasis of the transplanted tumor of transgenic lung cancer cell lines L9981-Luc in nude mice.