Construction and influence of human nuclear factor-κB p65 shRNA lentiviral 
vector on malignant biological behavior of lung cancer cells

• Published in: Zhongguo fei ai za zhi Vol. 17; no. 5; pp. 369 - 377
• Main Authors: Guo, Hongjuan, Zhu, Guangfa, Wu, Chunting
• Format: Journal Article
• Language: Chinese
• Published: China Chinese Anti-Cancer Association Chinese Antituberculosis Association 01.05.2014; Chinese Anti-Cancer Association; Chinese Antituberculosis Association
• Subjects: Adhesion; Cell adhesion & migration; Cell Line, Tumor; Cell Movement; Down-Regulation; Genetic Vectors - genetics; Genetic Vectors - metabolism; Humans; IκBα; Kinases; Lentiviral vector; Lentivirus - genetics; Lentivirus - metabolism; Lung cancer; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - pathology; Migration; NF-κBp65; Proteins; RNA Interference; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; Stable transfection cell line; Transcription Factor RelA - genetics; Transcription Factor RelA - metabolism; Transfection; Wu Yi; Beijing China; China
• ISSN: 1009-3419; 1999-6187
• DOI: 10.3779/j.issn.1009-3419.2014.05.02

Nuclear factor-κB is an important transcription factor and is closely associated with a variety of malignant tumors. The biological behavior of lung tumor cells can be reversed by inhibiting the expression of NF-κBp65 directly or indirectly. Nuclear factor-κBp65 gene shRNA recombinant plasmids were constructed and then infected with A549 cells. New stable cell lines were selected, and the ability of migration and adhesion was identified. Both scramble control sequence and interference sequence (shRNA) of human nuclear factor-κBp65 were designed and synthesized to build recombinant plasmids, with BamH I site at the 5' end and Xho I and EcoR I sites at the 3' end. A549 cells were infected, and stable transfection strains were selected by puromycin. Western blot and qRT-PCR methods were applied to assess the interference efficient of NF-κBp65 and the protein expression level of IκBα. Transwell and MTT assays were carried out to analyze the ability of migration and adhesion of A549 cells separately. Recombinant p