Clinical Assessment of Chemosensitivity Test in Xeno-free Culture of Autologous 
Malignant Effusion Cells from Patients with Advanced Lung Adenocarcinoma

• Published in: Zhongguo fei ai za zhi Vol. 19; no. 9; pp. 584 - 589
• Main Authors: Chen, Liang, Yang, Shunfang, Jiang, Jinqi, Zhang, Ying, Feng, Hui, Cao, Jie, Ge, Xinyue, Xie, Wenhui
• Format: Journal Article
• Language: Chinese
• Published: China Chinese Anti-Cancer Association Chinese Antituberculosis Association 20.09.2016; Chinese Anti-Cancer Association; Chinese Antituberculosis Association
• Subjects: Adenocarcinoma - drug therapy; Adenocarcinoma - mortality; Adenocarcinoma of Lung; Aged; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - pharmacology; Cell; Chemotherapy; Cisplatin - pharmacology; Ex vivo chemo-sensitivity assay; Female; Gastric cancer; Humans; Lung cancer; Lung neoplasms; Lung Neoplasms - drug therapy; Lung Neoplasms - mortality; Male; Malignant effusions; Middle Aged; Pleural Effusion, Malignant - cytology; Pleural Effusion, Malignant - drug therapy; Pleural Effusion, Malignant - mortality; Survival Rate; Tumor Cells, Cultured - drug effects; Xeno-free culture; China
• ISSN: 1009-3419; 1999-6187
• DOI: 10.3779/j.issn.1009-3419.2016.09.04

A great individual differences to chemotherapeutic effects existed in the patient with advanced lung cancer. How to choose the optimum regimens to achieve the individuation and maximum effect of chemotherapy for lung cancer is worth exploring. The study was designed to examine the effect of ex vitro chemo-sensitivity assay in xeno-free culture of autologous malignant effusion cells from patients with advanced lung adenocarcinoma. The 50 treatment-naive patients with lung adenocarcinoma complicated with malignant pleural or pericardial effusions were enrolled. Effusions of all cases had been controlled by closed drainage and 300 mL-500 mL of which were retained under sterile condition from 25 cases (Chemo-sensitivity group). Primary malignant effusion cells were isolated from autologous effusions of the patients. Then, xeno-free culture (average 11 days) were intervened with 8 chemotherapeutic drugs commonly used in clinical practice and were determined by CCK-8 assay. Optimum regimens were selected for chemot