Chronic granulomatous inflammation in teleost fish Piaractus mesopotamicus: histopathology model study

This study evaluated the cell kinetics and formation of granuloma during chronic inflammation induced by Bacillus Calmette-Guérin (BCG) in the skeletal muscle of Piaractus mesopotamicus, as a histopathology model to study innate immunity. Sixty fish were divided in two groups: BCG-inoculated and non...

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Bibliographic Details
Published inRevista MVZ Córdoba Vol. 22; no. 1; pp. 5738 - 5746
Main Authors Manrique, W G, Figueiredo, M A P, Belo, M A A, Martins, M L, Moraes, F R
Format Journal Article
LanguageEnglish
Portuguese
Published Monteria Universidad de Córdoba - Facultad de Medicina Veterinaria y Zootecnia 01.01.2017
Subjects
AGRICULTURE, DAIRY & ANIMAL SCIENCE
Bacterial diseases
BCG
Bio-assays
Freshwater
Freshwater fishes
granuloma
Histopathology
Immunity
macrophage
macrófago
micobactéria
mycobacterium
pacu (Fuente: CAB)
pacu (Source: CAB)
Piaractus mesopotamicus
pacu (Source: CAB)
macrófago
BCG
granuloma
micobactéria
macrophage, mycobacterium
pacu (Fuente: CAB)
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Summary:This study evaluated the cell kinetics and formation of granuloma during chronic inflammation induced by Bacillus Calmette-Guérin (BCG) in the skeletal muscle of Piaractus mesopotamicus, as a histopathology model to study innate immunity. Sixty fish were divided in two groups: BCG-inoculated and non-inoculated fish and the inflammatory response analyzed 3, 7, 14, 21 and 33 days post-inoculation (DPI) by histopathological analysis after hematoxylin-eosin and Ziehl-Neelsen staining. After 3 DPI of BCG showed a diffuse inflammatory reaction mostly composed by mononuclear cells. The inflammation continued diffuse 7 DPI initiating the cellular organization surrounding the inoculum and have continued at 14 DPI with discrete presence of epithelioid-like type cells with acidophilic cytoplasm and floppy chromatin. Higher cellular organization (21 DPI) surrounding the granuloma with intense peripheral mononuclear inflammatory infiltrate and nevertheless, an increase in the number of fibroblasts and macrophage-like cells was observed. The inflammatory process became less diffuse 33 DPI with formation of small amount of granuloma surrounded by the same type of reaction found in bigger granuloma. Both the young and old granuloma presented typical characteristic around the inoculum composed by a layer of epithelioid-like type cells, besides macrophages, some lymphocytes and abundant fibroblasts.
ISSN:0122-0268
1909-0544
1909-0544
DOI:10.21897/rmvz.933