Effect of silver nanoparticles on biofilm formation of Pseudomonas fluourcens

• Published in: Zhejiang da xue xue bao. Journal of Zhejiang University. Sciences edition. Li xue ban Vol. 43; no. 2; pp. 195 - 199
• Main Authors: Xunchen, Huang, Wei, Yang, Xianjun, Dai
• Format: Journal Article
• Language: Chinese
• Published: Zhejiang University Press 01.03.2016
• Subjects: Bacteria; Biofilms; Ethylene vinyl acetates; Formations; Mathematical analysis; Mortality; Nanoparticles; Pseudomonas; 生物膜; 纳米银; 荧光假单胞菌
• ISSN: 1008-9497
• DOI: 10.3785/j.issn.1008-9497.2016.02.013

To explore the effect of silver nanoparticles on the formation of Pseudomonas fluourcens biofilms on ethylene-vinyl acetate(EVA) pieces, the curve of bacterial biofilm is determined by colony counts, and the morphology, microstructure and bacterial status of the biofilm are respectively observed through crystal voilet staining, scanning electron microscopy(SEM) and confocal laser scanning microscope(CLSM).The biofilm stained by crystal violet is examined with a digital microscope to calculate the biofilm coverage rate (BCR) by NIS-Element BR soft ware. Then the mortality of bacterial is calculated by image structure analyzer (ISA) software.Results show that the bacteria can aggregate on the control EVA and EVA contained 0.4% silver nanoparticles, and forms the grown biofilm after 12 and 48 h culture, respectively. But the biofilm on the control EVA has more bacterial count and BCR than that of silver nanoparticles(p<0.05). At 24 h, the bacteria interacting with its extracellular products can form the dense fi