THE ALKALOID-FREE FRACTION FROM Galega officinalis EXTRACT PREVENTS OXIDATIVE STRESS UNDER EXPERIMENTAL DIABETES MELLITUS

• Published in: Ukrainian biochemical journal Vol. 87; no. 4; pp. 78 - 86
• Main Authors: Lupak, M I, Khokhla, M R, Hachkova, G Ya, Kanyuka, O P, Klymyshyn, N I, Chajka, Ya P, Skybitska, M I, Sybirna, N O
• Format: Journal Article
• Language: Ukrainian; English
• Published: Ukraine National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry 01.07.2015
• Subjects: alkaloid-free fraction from Galega officinalis extract; Animals; Catalase - blood; Diabetes Mellitus, Experimental - chemically induced; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - enzymology; Diabetes Mellitus, Experimental - pathology; Erythrocytes - drug effects; Erythrocytes - enzymology; Erythrocytes - pathology; experimental diabetes; Flavonoids; Galega - chemistry; Glutathione Peroxidase - blood; Hypoglycemic Agents - isolation & purification; Hypoglycemic Agents - pharmacology; Leukocytes, Mononuclear - drug effects; Leukocytes, Mononuclear - enzymology; Leukocytes, Mononuclear - pathology; Lipid Peroxidation - drug effects; Male; oxidative stress; Oxidative Stress - drug effects; Phytol; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Rats; Reactive Oxygen Species - antagonists & inhibitors; Reactive Oxygen Species - metabolism; Streptozocin; Superoxide Dismutase - blood
• ISSN: 2409-4943; 2413-5003
• DOI: 10.15407/ubj87.04.078

The effect of alkaloid-free fraction from Galega officinalis extract on the process of formation of reactive oxygen species and indicators of prooxidant-antioxidant balance was investigated in rat peripheral blood under conditions of experimental diabetes mellitus. It was shown that alkaloid-free fraction from Galega officinalis extract prevents oxidative stress development in rats with streptozotocin-induced diabetes, providing antioxidant and antiradical mobilization mechanisms to protect the blood system. In the case of extract application to animals with studied pathology, one can observe a reducing effect of reactive oxygen species generation in leukocytes, inhibition of proteins and lipids oxidative modification processes and increased activity of key enzymes of rat peripheral blood antioxidant system (superoxide dismutase, catalase and glutathione peroxidase). The revealed biological effect could be explained by the presence of biologically active substances with antioxidant properties in the extract composition (phytol and flavonoids).