Caracterização do gene vip3A e toxicidade da proteína Vip3Aa50 à lagarta-do-cartucho e à lagarta-da-soja

O objetivo deste trabalho foi caracterizar o gene vip3A de Bacillus thuringiensis e verificar a toxicidade da proteína Vip3Aa50 a larvas da lagarta-do-cartucho (Spodoptera frugiperda) e da lagarta-da-soja (Anticarsia gemmatalis). O gene vip3A foi amplificado por PCR, com iniciadores específicos, e g...

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Published inPesquisa agropecuaria brasileira Vol. 48; no. 9; pp. 1220 - 1227
Main Authors Figueiredo, Camila Soares, Marucci, Suzana Cristina, Tezza, Renata Izabel Dozzi, Lemos, Manoel Victor Franco, Desidério, Janete Apparecida
Format Journal Article
LanguageEnglish
Portuguese
Published Embrapa Secretaria de Pesquisa e Desenvolvimento; Pesquisa Agropecuária Brasileira 01.09.2013
Embrapa Informação Tecnológica
Subjects
AGRICULTURE, DAIRY & ANIMAL SCIENCE
AGRICULTURE, MULTIDISCIPLINARY
Anticarsia gemmatalis
controle biológico
expressão heteróloga
proteína inseticida vegetativa
Spodoptera frugiperda
vegetative insecticidal protein
controle biológico
proteína inseticida vegetativa
Anticarsia gemmatalis
Spodoptera frugiperda
expressão heteróloga
biological control
heterologous expression
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Abstract O objetivo deste trabalho foi caracterizar o gene vip3A de Bacillus thuringiensis e verificar a toxicidade da proteína Vip3Aa50 a larvas da lagarta-do-cartucho (Spodoptera frugiperda) e da lagarta-da-soja (Anticarsia gemmatalis). O gene vip3A foi amplificado por PCR, com iniciadores específicos, e gerou um fragmento de 2.370 pb. Esse fragmento foi clonado em vetor pGEM-T Easy e, em seguida, sequenciado, subclonado em vetor de expressão pET-28a (+) e inserido em células de Escherichia coli BL21 (DE3). A expressão da proteína Vip3Aa50 foi induzida por isopropil-β-D-1-tiogalactopiranosídeo (IPTG), visualizada em SDS-PAGE e detectada por "Western blot". Os ensaios de toxicidade revelaram alta atividade da proteína Vip3Aa50 contra as larvas neonatas da lagarta-da-soja e da lagarta-do-cartucho, com CL50 de 20,3 e 79,6 ng cm-2, respectivamente. O gene vip3Aa50 é um novo gene da classe vip3A.
AbstractList O objetivo deste trabalho foi caracterizar o gene vip3A de Bacillus thuringiensis e verificar a toxicidade da proteína Vip3Aa50 a larvas da lagarta-do-cartucho (Spodoptera frugiperda) e da lagarta-da-soja (Anticarsia gemmatalis). O gene vip3A foi amplificado por PCR, com iniciadores específicos, e gerou um fragmento de 2.370 pb. Esse fragmento foi clonado em vetor pGEM-T Easy e, em seguida, sequenciado, subclonado em vetor de expressão pET-28a (+) e inserido em células de Escherichia coli BL21 (DE3). A expressão da proteína Vip3Aa50 foi induzida por isopropil-β-D-1-tiogalactopiranosídeo (IPTG), visualizada em SDS-PAGE e detectada por "Western blot". Os ensaios de toxicidade revelaram alta atividade da proteína Vip3Aa50 contra as larvas neonatas da lagarta-da-soja e da lagarta-do-cartucho, com CL50 de 20,3 e 79,6 ng cm-2, respectivamente. O gene vip3Aa50 é um novo gene da classe vip3A.
Author Tezza, Renata Izabel Dozzi
Desidério, Janete Apparecida
Lemos, Manoel Victor Franco
Marucci, Suzana Cristina
Figueiredo, Camila Soares
AuthorAffiliation Universidade Estadual Paulista
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  givenname: Camila Soares
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DocumentTitleAlternate Characterization of the vip3A gene and toxicity of Vip3Aa50 protein to fall armyworm and velvetbean caterpillar
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Issue 9
Keywords vegetative insecticidal protein
controle biológico
proteína inseticida vegetativa
Anticarsia gemmatalis
Spodoptera frugiperda
expressão heteróloga
biological control
heterologous expression
Language English
Portuguese
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References LIU, J.; SONG, F.; ZHANG, J.; LIU, R.; HE, K.; TAN, J.; HUANG, D 2007; 45
ESTRUCH, J.J.; WARREN, G.W.; MULLINS, M.A.; NYE, G.J.; CRAIG, J.A.; KOZIEL, M.G 1996; 93
Warren, G.W; CAROZZ, I, N.B; KOZIEL, M 1997
FANG, J.; XU, X.; WANG, P.; ZHAO, J.Z.; SHELTON, A.M.; CHENG, J.; FENG, M.G.; SHEN, Z 2007; 73
GORDON, D.; ABAJIAN, C.; GREEN, P 1998; 8
FIÚZA, L.M.; BERLIT Z, D.L 2009
CHAKROUN, M.; BEL, Y.; CACCIA, S.; ABDELKEFI-MESRATI, L.; ESCRICHE, B.; FERRÉ, J. 2012; 110
MENDES, S.M.; MARUCCI, R.C.; MOREIRA, S.G.; WAQUIL, J.M 2008
SHEN, J.; HOU, M.; GUO, W 2009; 49
HANAHAN, D 1983; 166
SAMPURNA, S.; MAITI, M.K 2011; 21
EWING, B.; GREEN, P 1998; 8
LEE, M.K.; MILES, P.; CHEN, J.S 2006; 339
TOWBIN, H.; STAEHETIN, A.; GORDON, J 1979; 76
SANGER, F.; NICKLEN, S.; COULSON, A.R 1977; 74
MONNERAT, R.; BRAVO, A; MELO, I.S.; AZEVEDO, J.L. 2000
SELVAPANDIYAN, A.; ARORA, N.; RAJAGOPAL, R.; JALALI, S.K.; VEN KATESAN, T.; SINGH, S.P.; BHATNAGAR, R.K 2001; 67
WU, J.; ZHAO, F.; BAI, J.; DENG, G.; QIN, S.; BAO, Q 2007; 34
CRICKMORE, N; ZEIGLER, D.R; SCHNEPF, E.; VAN RIE, J.; LERECLUS, D; BAUM, J.; BRAVO, A.; DEAN, D.H
Ewing, B.; Hillier, L.; Wendl, M.C.; Green, P 1998; 8
SAUKA, D.H.; RODRIGUE, Z, S.E.; BENINTENDE, G.B 2013; 22
PRAÇA, L.B.; BATISTA, A.C.; MARTINS, É.S.; SIQUEIRA, C.B.; DIAS, D.G. de S.; GOMES, A.C.M.M.; FALCÃO, R.; MONNERAT, R.G 2004; 39
GOBATTO, V.; GIANI, S.G; CAMASSOLA, M.; DILLON, A.J.P.; SPECHT, A.; BARROS, N.M 2010; 70
LAEMMLI, U.K 1970; 227
MENDONÇA, E.G. DE; VISÔTTO, L.E.; COSTA, N.C.S.; RIBEIRO, F.R.; OLIVEIRA, J.A. de; OLIVEIRA, M.G. de A 2011; 35
FERRÉ, J.; VAN RIE, J 2002; 47
LOGUERCIO, L.L.; BARRETO, M.L.; ROCHA, T.L.; SANTOS, C.G.; TEIXEIRA, F.F.; PAIVA, E 2002; 93
CRICKMORE, N.; ZEIGLER, D.R.; FEITELSON, J.; SCHNEPF, E.; VAN RIE, J.; LERECLUS, D.; BAUM, J.; DEAN, D.H 1998; 62
FIUZA, L.M.; SCHÜ ENEMANN, R; PINTO, L.M.N.; ZANETTINI, M.H.B 2012; 72
MARUCCI, S.C 2010
SENA, J.A.D.; HERNANDEZ-RODRIGUES, C.S.; FERRE, J 2009; 75
2013
ABDEL KEFI -MESRATI, L.; BOU KEDI, H.; CHA KROUN, M.; KAMOUN, F.; AZZOU, Z, H.; TOUNSI, S.; ROUIS, S.; JAOUA, S 2011; 107
BERGAMASCO, V.B.; MENDES, D.R.P.; FERNANDES, O.A.; DESIDÉRIO, J.A.; LEMOS, M.V.F 2013; 112
STUDIER, F.W.; MOFFATT, B.A 1986; 189
LETOWSKI, J.; BRAVO, A.; BROUSSEAU, R.; MASSON, L 2005; 71
SAMBROOK, J.; RUSSEL, D.W 2001
References_xml – volume: 72
  start-page: 363
  year: 2012
  end-page: 369
  article-title: Two new Brazilian isolates of Bacillus thuringiensis toxic to Anticarsia gemmatalis (Lepidoptera: Noctuidae)
  publication-title: Brazilian Journal of Biology
  contributor:
    fullname: FIUZA, L.M.; SCHÜ ENEMANN, R; PINTO, L.M.N.; ZANETTINI, M.H.B
– volume: 45
  start-page: 432
  year: 2007
  end-page: 438
  article-title: Identification of vip3A-type genes from Bacillus thuringiensis strains and characterization of a novel vip3A-type gene
  publication-title: Letters in Applied Microbiology
  contributor:
    fullname: LIU, J.; SONG, F.; ZHANG, J.; LIU, R.; HE, K.; TAN, J.; HUANG, D
– volume: 21
  start-page: 937
  year: 2011
  end-page: 946
  article-title: Molecular characterization of a novel vegetative insecticidal protein from Bacillus thuringiensis effective against sap-sucking insect pest
  publication-title: Journal of Microbiology and Biotechnology
  contributor:
    fullname: SAMPURNA, S.; MAITI, M.K
– volume: 49
  start-page: 110
  year: 2009
  end-page: 116
  article-title: Identification and cloning of vip3A genes from isolates of Bacillus thuringiensis and their bioactivity analysis
  publication-title: Wei Sheng Wu Xue Bao
  contributor:
    fullname: SHEN, J.; HOU, M.; GUO, W
– volume: 76
  start-page: 4350
  year: 1979
  end-page: 4354
  article-title: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications
  publication-title: Proceedings of the National Academy of Sciences
  contributor:
    fullname: TOWBIN, H.; STAEHETIN, A.; GORDON, J
– volume: 47
  start-page: 501
  year: 2002
  end-page: 533
  article-title: Biochemistry and genetics of insect resistance to Bacillus thuringiensis
  publication-title: Annual Review of Entomology
  contributor:
    fullname: FERRÉ, J.; VAN RIE, J
– volume: 70
  start-page: 1039
  year: 2010
  end-page: 1046
  article-title: Bacillus thuringiensis isolates entomopathogenic for Culex quinquefasciatus (Diptera: Culicidae) and Anticarsia gemmatalis (Lepidoptera: Noctuidae)
  publication-title: Brazilian Journal of Biology
  contributor:
    fullname: GOBATTO, V.; GIANI, S.G; CAMASSOLA, M.; DILLON, A.J.P.; SPECHT, A.; BARROS, N.M
– volume: 110
  start-page: 334
  year: 2012
  end-page: 339
  article-title: Susceptibility of Spodoptera frugiperda and S. exigua to Bacillus thuringiensis Vip3Aa insecticidal protein
  publication-title: Journal of Invertebrate Pathology
  contributor:
    fullname: CHAKROUN, M.; BEL, Y.; CACCIA, S.; ABDELKEFI-MESRATI, L.; ESCRICHE, B.; FERRÉ, J.
– volume: 227
  start-page: 680
  year: 1970
  end-page: 681
  article-title: Cleavage of structural proteins during assembly of the head of bacteriophage T4
  publication-title: Nature
  contributor:
    fullname: LAEMMLI, U.K
– start-page: 163
  year: 2000
  end-page: 200
  publication-title: Controle biológico
  contributor:
    fullname: MONNERAT, R.; BRAVO, A; MELO, I.S.; AZEVEDO, J.L.
– start-page: 109
  year: 1997
  end-page: 121
  publication-title: Advances in insect control: the role of transgenic plants. London
  contributor:
    fullname: Warren, G.W; CAROZZ, I, N.B; KOZIEL, M
– volume: 75
  start-page: 2236
  year: 2009
  end-page: 2237
  article-title: Interaction of Bacillus thuringiensis Cry1 and Vip3A proteins with Spodoptera frugiperda midgut binding sites
  publication-title: Applied and Environmental Microbiology
  contributor:
    fullname: SENA, J.A.D.; HERNANDEZ-RODRIGUES, C.S.; FERRE, J
– year: 2008
  publication-title: Milho Bt: avaliação preliminar da resistência de híbridos comerciais à lagarta-do-cartucho, Spodoptera frugiperda (J. E. Smith, 1797)
  contributor:
    fullname: MENDES, S.M.; MARUCCI, R.C.; MOREIRA, S.G.; WAQUIL, J.M
– volume: 166
  start-page: 557
  year: 1983
  end-page: 580
  article-title: Studies on transformation of Escherichia coli with plasmids
  publication-title: Journal of Molecular Biology
  contributor:
    fullname: HANAHAN, D
– volume: 93
  start-page: 5389
  year: 1996
  end-page: 5394
  article-title: Vip3A, a novel Bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects
  publication-title: Proceedings of the National Academy of Sciences of the United States of America
  contributor:
    fullname: ESTRUCH, J.J.; WARREN, G.W.; MULLINS, M.A.; NYE, G.J.; CRAIG, J.A.; KOZIEL, M.G
– start-page: 58
  issue: 38
  year: 2009
  end-page: 60
  article-title: Produtos de Bacillus thuringiensis, registro e comercialização
  publication-title: Biotecnologia Ciência e Desenvolvimento
  contributor:
    fullname: FIÚZA, L.M.; BERLIT Z, D.L
– volume: 93
  start-page: 269
  year: 2002
  end-page: 277
  article-title: Combined analysis of supernatant-based feeding bioassays and PCR as a first-tier screening strategy for Vip-derived activities in Bacillus thuringiensis strains effective against tropical fall armyworm
  publication-title: Journal of Applied Microbiology
  contributor:
    fullname: LOGUERCIO, L.L.; BARRETO, M.L.; ROCHA, T.L.; SANTOS, C.G.; TEIXEIRA, F.F.; PAIVA, E
– year: 2001
  publication-title: Molecular cloning: a laboratory manual
  contributor:
    fullname: SAMBROOK, J.; RUSSEL, D.W
– volume: 107
  start-page: 198
  year: 2011
  end-page: 201
  article-title: Investigation of the steps involved in the difference of susceptibility of Ephestia kuehniella and Spodoptera littoralis to the Bacillus thuringiensis Vip3Aa16 toxin
  publication-title: Journal of Invertebrate Pathology
  contributor:
    fullname: ABDEL KEFI -MESRATI, L.; BOU KEDI, H.; CHA KROUN, M.; KAMOUN, F.; AZZOU, Z, H.; TOUNSI, S.; ROUIS, S.; JAOUA, S
– volume: 112
  start-page: 152
  year: 2013
  end-page: 158
  article-title: Bacillus thuringiensis Cry1Ia10 and Vip3Aa protein interactions and their toxicity in Spodoptera spp. (Lepidoptera).
  publication-title: Journal of Invertebrate Pathology
  contributor:
    fullname: BERGAMASCO, V.B.; MENDES, D.R.P.; FERNANDES, O.A.; DESIDÉRIO, J.A.; LEMOS, M.V.F
– volume: 8
  start-page: 195
  year: 1998
  end-page: 202
  article-title: Consed: a graphical tool for sequence finishing
  publication-title: Genome Research
  contributor:
    fullname: GORDON, D.; ABAJIAN, C.; GREEN, P
– publication-title: Bacillus thuringiensis toxin nomenclature
  contributor:
    fullname: CRICKMORE, N; ZEIGLER, D.R; SCHNEPF, E.; VAN RIE, J.; LERECLUS, D; BAUM, J.; BRAVO, A.; DEAN, D.H
– volume: 8
  start-page: 175
  year: 1998
  end-page: 185
  article-title: Base-calling of automated sequencer traces using phred: I. Accuracy assessment
  publication-title: Genome Research
  contributor:
    fullname: Ewing, B.; Hillier, L.; Wendl, M.C.; Green, P
– volume: 67
  start-page: 5855
  year: 2001
  end-page: 5858
  article-title: Toxicity analysis of N- and C-terminus-deleted vegetative insecticidal protein from Bacillus thuringiensis
  publication-title: Applied and Environmental Microbiology
  contributor:
    fullname: SELVAPANDIYAN, A.; ARORA, N.; RAJAGOPAL, R.; JALALI, S.K.; VEN KATESAN, T.; SINGH, S.P.; BHATNAGAR, R.K
– volume: 339
  start-page: 1043
  year: 2006
  end-page: 1047
  article-title: Brush border membrane binding properties of Bacillus thuringiensis Vip3A toxin to Heliothis virescens and Helicoverpa zea midguts
  publication-title: Biochemical and Biophysical Research Communications
  contributor:
    fullname: LEE, M.K.; MILES, P.; CHEN, J.S
– volume: 62
  start-page: 807
  year: 1998
  end-page: 813
  article-title: Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins
  publication-title: Microbiology and Molecular Biology Reviews
  contributor:
    fullname: CRICKMORE, N.; ZEIGLER, D.R.; FEITELSON, J.; SCHNEPF, E.; VAN RIE, J.; LERECLUS, D.; BAUM, J.; DEAN, D.H
– volume: 22
  start-page: 373
  year: 2013
  end-page: 380
  article-title: New variants of lepidoptericidal toxin genes encoding Bacillus thuringiensis Vip3Aa proteins
  publication-title: Journal of Molecular Microbiology and Biotechnology
  contributor:
    fullname: SAUKA, D.H.; RODRIGUE, Z, S.E.; BENINTENDE, G.B
– volume: 189
  start-page: 113
  year: 1986
  end-page: 130
  article-title: Use of bacteriophage T-7 RNA polymerase to direct selective high-level expression of cloned genes
  publication-title: Journal of Molecular Biology
  contributor:
    fullname: STUDIER, F.W.; MOFFATT, B.A
– volume: 74
  start-page: 5463
  year: 1977
  end-page: 5467
  article-title: DNA sequencing with chain-terminating inhibitors
  publication-title: Proceedings of the National Academy of Sciences
  contributor:
    fullname: SANGER, F.; NICKLEN, S.; COULSON, A.R
– year: 2013
  publication-title: GenBank
– volume: 39
  start-page: 11
  year: 2004
  end-page: 16
  article-title: Estirpes de Bacillus thuringiensis efetivas contra insetos das ordens Lepidoptera, Coleoptera e Diptera
  publication-title: Pesquisa Agropecuária Brasileira
  contributor:
    fullname: PRAÇA, L.B.; BATISTA, A.C.; MARTINS, É.S.; SIQUEIRA, C.B.; DIAS, D.G. de S.; GOMES, A.C.M.M.; FALCÃO, R.; MONNERAT, R.G
– volume: 73
  start-page: 956
  year: 2007
  end-page: 961
  article-title: Characterization of chimeric Bacillus thuringiensis Vip3 toxins
  publication-title: Applied and Environmental Microbiology
  contributor:
    fullname: FANG, J.; XU, X.; WANG, P.; ZHAO, J.Z.; SHELTON, A.M.; CHENG, J.; FENG, M.G.; SHEN, Z
– volume: 35
  start-page: 446
  year: 2011
  end-page: 454
  article-title: Caracterização enzimática de isoformas de cisteíno protease de Anticarsia gemmatalis (HÜBNER, 1818)
  publication-title: Ciência e Agrotecnologia
  contributor:
    fullname: MENDONÇA, E.G. DE; VISÔTTO, L.E.; COSTA, N.C.S.; RIBEIRO, F.R.; OLIVEIRA, J.A. de; OLIVEIRA, M.G. de A
– volume: 34
  start-page: 649
  year: 2007
  end-page: 660
  article-title: Evidence for positive Darwinian selection of Vip gene in Bacillus thuringiensis
  publication-title: Journal of Genetics and Genomics
  contributor:
    fullname: WU, J.; ZHAO, F.; BAI, J.; DENG, G.; QIN, S.; BAO, Q
– volume: 71
  start-page: 5391
  year: 2005
  end-page: 5398
  article-title: Assessment of cry1 gene contents of Bacillus thuringiensis strains by use of DNA microarrays
  publication-title: Applied and Environmental Microbiology
  contributor:
    fullname: LETOWSKI, J.; BRAVO, A.; BROUSSEAU, R.; MASSON, L
– volume: 8
  start-page: 186
  year: 1998
  end-page: 194
  article-title: Base-calling of automated sequencer traces using phred: II - Error probabilities
  publication-title: Genome Research
  contributor:
    fullname: EWING, B.; GREEN, P
– year: 2010
  publication-title: Seleção e caracterização de novos genes vip3A: genes inseticidas de segunda geração de Bacillus thuringiensis
  contributor:
    fullname: MARUCCI, S.C
SSID ssj0027367
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Snippet O objetivo deste trabalho foi caracterizar o gene vip3A de Bacillus thuringiensis e verificar a toxicidade da proteína Vip3Aa50 a larvas da lagarta-do-cartucho...
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SubjectTerms AGRICULTURE, DAIRY & ANIMAL SCIENCE
AGRICULTURE, MULTIDISCIPLINARY
Anticarsia gemmatalis
controle biológico
expressão heteróloga
proteína inseticida vegetativa
Spodoptera frugiperda
Title Caracterização do gene vip3A e toxicidade da proteína Vip3Aa50 à lagarta-do-cartucho e à lagarta-da-soja
URI http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000900005&lng=en&tlng=en
https://doaj.org/article/0bbc2234128b4c9790bb2077edd26524
Volume 48
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