Exo70 Enhances Exosome Secretion by Modulating Multivesicular Body Trafficking to Promote Pancreatic Cancer Metastasis

Objective　To investigate the role and molecular mechanisms of Exo70 in the metastasis of pancreatic cancer cells. Methods　Stable cell lines with Exo70 knockdown or overexpression were established using lentiviral infection. The migration ability of pancreatic cancer cells was assessed by Transwell a...

Full description

Saved in:
Bibliographic Details
Published inZhongliu fangzhi yanjiu Vol. 52; no. 7; pp. 585 - 591
Main Authors Zhang, Wenqing, Xiang, Jingzhou, Hu, Tianhui
Format Journal Article
LanguageChinese
English
Published Tianjin China Anti-Cancer Association 2025
Magazine House of Cancer Research on Prevention and Treatment
Subjects
Accumulation
Cancer
CD63 antigen
Cell migration
Degradation
exo70
Exosomes
Immunofluorescence
Localization
Lysosomes
Metastases
Metastasis
Molecular modelling
Pancreatic cancer
Transmission electron microscopy
Tumor cell lines
Online AccessGet full text

Cover

More Information
Summary:Objective　To investigate the role and molecular mechanisms of Exo70 in the metastasis of pancreatic cancer cells. Methods　Stable cell lines with Exo70 knockdown or overexpression were established using lentiviral infection. The migration ability of pancreatic cancer cells was assessed by Transwell assay. The morphology, particle size, and secretion levels of exosomes were observed using transmission electron microscopy. Changes in the expression of Exo70 and exosomal marker proteins were detected by Western blot. The localization of multivesicular bodies (MVBs) was examined by immunofluorescence. Results　Exo70 knockdown inhibited the migration ability of pancreatic cancer cells and substantially reduced the total amount of exosome secretion. By contrast, Exo70 overexpression enhanced the migration ability of pancreatic cancer cells. Immunofluorescence results showed that Exo70 knockdown induced the perinuclear accumulation of CD63 and LAMP2, leading to an increase in MVB accumulation and lysosomal degradation in pancreatic cancer cells. Conclusion　Exo70 promotes the normal transport of MVBs and maintains exosome secretion. Its knockdown enhances the lysosomal degradation of MVBs, resulting in impaired exosome biogenesis and secretion and thereby inhibiting the migration of pancreatic cancer cells.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
ISSN:1000-8578
DOI:10.3971/j.issn.1000-8578.2025.25.0232