Effects of vibration on expressions of vascular endothelial inflammatory factors and IncRNA MEG3 in vitro

• Published in: Huan jing yu zhi ye yi xue = Journal of environmental & occupational medicine Vol. 39; no. 11; pp. 1209 - 1213
• Main Authors: Yang, Hongyu, Chen, Qingsong, Li, Zheng, Chen, Ziyu, Du, Bingqian, Zhang, Xi, Hu, Xiuwen, Xia, Yun
• Format: Journal Article
• Language: Chinese; English
• Published: Shanghai Shanghai Municipal Center For Disease Control and Prevention 2022; Editorial Committee of Journal of Environmental and Occupational Medicine
• Subjects: Cell viability; Cholecystokinin; Cytokines; Endothelial cells; Enzyme-linked immunosorbent assay; Exposure; Fluorescence; Frequencies; human umbilical vein endothelial cells; In vitro methods and tests; Inflammation; inflammatory factor; Inflammatory response; Injuries; Interleukin 10; Interleukin 4; Interleukin 8; maternally expressed 3; Non-coding RNA; Statistical analysis; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Umbilical vein; Vibration; Vibration effects
• ISSN: 2095-9982
• DOI: 10.11836/JEOM22167

[Background] Prolonged exposure to vibration can cause vascular endothelial injury, and inflammatory response plays an important role in vascular endothelial injury. Studies have shown that long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) is involved in regulating the expression of inflammatory injury of endothelial cells. [Objective] To investigate the effects of vibration on the secretion of inflammatory factors and the expression of IncRNA MEG3 by vascular endothelial cells in vitro. [Methods] Human umbilical vein endothelial cells (HUVEC) were divided into two categories: vibration and control. The vibration exposure included 63 Hz (6.76 m·s−2), 200 Hz (5.08 m·s−2), and 250 Hz (4.56 m·s−2) frequency bands, and 1 and 2 d exposure time with 1 to 4 h of daily vibration. The control treatment was the same as the vibration category except that they were not exposed to vibration. CCK-8 was used to detect the effects of different vibration frequencies and time on the viability of HUVEC. The expression levels of tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8), interleukin-4 (IL-4), and interleukin-10 (IL-10) in the cells and supernatants were detected by enzyme-linked immunosorbent assay. The expression levels of IncRNA MEG3 were detected by real-time fluorescence quantitative PCR. [Results] Compared with the cells with the control treatment, the cell viability of the 1-day exposure group increased after 1.5 h and 3 h of vibration at 63 Hz, while decreased after 2 h and 2.5 h; the cell viability of the 2-day exposure group increased at the frequency of 63 Hz for 1.5 h, but decreased at 2 h and 2.5 h. At the frequency of 200 Hz, the cell viability of the 1-day exposure group increased at 2 h and 4 h, but decreased at 2.5 h and 3 h; the cell viability of the 2-day exposure group increased at 1.5 h and decreased at 2.5 h. For the vibration exposure at frequency of 250 Hz, the cell viability of the 1-day exposure group increased at 1.5 h and 2.5 h, but decreased at 3 h; of the 2-day exposure group, the cell viability increased at 1.5 h and decreased at 3 h. For the exposure settings of 63 and 200 Hz vibration for 2.5 h and 250 Hz vibration for 3 h, and with the control treatment as reference, the expression levels of TNF-α, IL-8, IL-4, and IL-10 in cells and supernatants were increased in the 1 d and 2 d exposures; the expression level of lncRNA MEG3 decreased in the 1 d exposure group; however, for the 2 d exposure, the expression level of lncRNA MEG3 decreased only in the 63 Hz vibration exposure. All of these results were statistically significant (P<0.05). [Conclusion] Vibration could induce an increase in the levels of inflammatory factors TNF-α, IL-8, IL-4, and IL-10 and a decrease in the expression level of lncRNA MEG3 in vascular endothelial cells in vitro.