LncRNA PCAT-1 Reduces Sensitivity of Cervical Carcinoma Cells to Cisplatin by Regulating STAT3 and PTEN Expression

• Published in: Zhongliu fangzhi yanjiu Vol. 48; no. 9; pp. 833 - 838
• Main Authors: Mao, Xuebao, Wang, Xiuhong
• Format: Journal Article
• Language: Chinese
• Published: Tianjin China Anti-Cancer Association 01.09.2021; Magazine House of Cancer Research on Prevention and Treatment
• Subjects: Cancer; Cell cycle; Cell viability; Cervical cancer; cervical cancer cells; Cervical carcinoma; Cervix; Cholecystokinin; Cisplatin; ddp; Flow cytometry; lncrnas; pcat-1; Protein expression; Proteins; PTEN protein; Regulatory mechanisms (biology); S phase; Sensitivity; Stat3 protein; Tumor cell lines
• ISSN: 1000-8578
• DOI: 10.3971/j.issn.1000-8578.2021.21.0148

Objective To investigate the role and regulatory mechanism of lncRNAs PCAT-1 in the sensitivity of cervical cancer cells to DDP. Methods The expressions of PCAT-1 in human cervical cancer cell lines (HeLa and SiHa) and DDP-resistant cell lines (HeLa/DDP and SiHa/DDP) were analyzed by realtime PCR. After PCAT-1 silencing and overexpression in HeLa/DDP and SiHa/DDP cells, CCK-8 and flow cytometry were used to detect cell viability ability and cell cycle, respectively. Western blot was used to detect the protein expression of STAT3 and PTEN. Results The DDP resistance index of HeLa/DDP cells to HeLa cells was 4.49, while that of SiHa/DDP cells to SiHa cells was 6.87. The expression levels of PCAT-1 in HeLa/DDP and SiHa/DDP cells were significantly higher than those in HeLa and SiHa cells, respectively (P<0.05). The overexpression of PCAT-1 reduced the sensitivity of HeLa/DDP and SiHa/DDP cells to DDP, enhanced the proportion of S phase in cell cycle, and decreased the proportion of G0-G1 and G2-M phases (P<0.05)