Dendrobium nobile active ingredient Dendrobin A against hepatocellular carcinoma via inhibiting nuclear factor kappa-B signaling

• Published in: Biomedicine & pharmacotherapy Vol. 177; p. 117013
• Main Authors: Yu, Yaping, Fan, Yonghao, Mei, Wenli, Xu, Xiaoqing, Chen, Yan, Zhao, Yangyang, Ruan, Banzhan, Shen, Zhihua, Lu, Yanda, Zheng, Shaojiang, Jie, Wei
• Format: Journal Article
• Language: English
• Published: Elsevier Masson SAS 01.08.2024; Elsevier
• Subjects: Biological function; Dendrobin A; Hepatocellular carcinoma; mRNA sequencing; NF-κB pathway; Hepatocellular carcinoma; Dendrobin A; Biological function; mRNA sequencing; NF-κB pathway
• ISSN: 0753-3322; 1950-6007; 1950-6007
• DOI: 10.1016/j.biopha.2024.117013

Dendrobin A, a typical active ingredient of the traditional Chinese medicine Dendrobium nobile, has potential clinical application in cancer treatment; however, its effect and mechanism in anti-hepatocellular carcinoma (HCC) remain unsolved. The effects of Dendrobin A on the viability, migration, invasion, cycle, apoptosis, and epithelial-mesenchymal transition of HepG2 and SK-HEP-1 cells were verified by in vitro experiments. mRNA sequencing was performed to screen the differentially expressed genes (DEGs) of HCC cells before and after Dendrobin A treatment, following GO enrichment and KEGG signaling pathway analyses. Mechanistically, molecular docking was used to evaluate the binding of Dendrobin A with proteins p65 and p50, before further verifying the activation of nuclear factor kappa-B (NF-κB) signaling. Finally, the antiproliferative effect of Dendrobin A on HCC cells was explored through animal experiments. Dendrobin A arrested cell cycle, induced apoptosis, and inhibited proliferation, migration, invasion, and blocked epithelial-mesenchymal transition in HepG2 and SK-HEP-1 cells. mRNA sequencing identified 830 DEGs, involving various biological processes. KEGG analysis highlighted NF-κB signaling. Molecular docking revealed strong binding of Dendrobin A with p65 and p50 proteins, and western blotting confirmed reduced levels of p-p65 and p-p50 in HCC cells post Dendrobin A treatment. NF-κB agonist PMA reversed Dendrobin A-inhibited cell proliferation migration and invasion. In vivo experiments showed that Dendrobin A inhibited HCC cell growth. Our findings suggest that Dendrobin A exhibits anti-HCC properties by inhibiting the activation of the NF-κB pathway. These results provide a scientific basis for utilizing Dendrobium nobile in anti-HCC therapies. [Display omitted] •Dendrobine A from Dendrobium nobile shows promise as an anti-HCC agent due to its multiple anti-cancer properties.•Dendrobine A inhibits the proliferation of HCC cells by arresting the cell cycle at the G2-M phase.•Dendrobine A induces apoptosis and inhibits the epithelial-mesenchymal transition of HCC cells.•The anti-HCC effects of Dendrobine A are related to its inhibition of the activation of NF-κB signaling.