Protective Effect of 6′′′-Feruloylspinosin on Aβ1-42-Induced SH-SY5Y Cells Injury

Objective: To investigate the protective effects of 6′′′-feruloylspinosinon Aβ1-42-induced SH-SY5Y cells injury. Methods: SH-SY5Y cells were pretreated with 6′′′-feruloylspinosin (1、5、10、20 and 40 μmol/L) for 2 h and then co-treated with 5 μmol/L of Aβ1-42 for 24 h. Cell viability was measured by CC...

Full description

Saved in:
Bibliographic Details
Published inShipin gongye ke-ji Vol. 43; no. 13; pp. 373 - 380
Main Authors Mei ZHANG, Yanqing ZHANG, Junbo XIE, Qing WANG, Wuqin QI
Format Journal Article
LanguageChinese
Published The editorial department of Science and Technology of Food Industry 01.07.2022
Subjects
6′′′-feruloylspinosin
amyloid β
antioxidant activity
mitochondria
ziziphi spinosae semen
Online AccessGet full text

Cover

More Information
Summary:Objective: To investigate the protective effects of 6′′′-feruloylspinosinon Aβ1-42-induced SH-SY5Y cells injury. Methods: SH-SY5Y cells were pretreated with 6′′′-feruloylspinosin (1、5、10、20 and 40 μmol/L) for 2 h and then co-treated with 5 μmol/L of Aβ1-42 for 24 h. Cell viability was measured by CCK-8 assay; cell morphology was observed by Calcein-AM/PI assay; intracellular reactive oxygen species, malonic dialdehyde level, Glutathione peroxidaseactivity and mitochondrial membrane potential level were detected by the kit; expression of apoptosis-related proteins: Bcl-xL/Bcl-2 associated death promoter, B-cell lymphoma/leukemia-2 were detected by Western Blotting. Results: 6′′′-feruloylspinosin could inhibit Aβ1-42-induced apoptosis and improve cell viability (P<0.001); reduce intracellular reactive oxygen species (P<0.001) and malondialdehyde (P<0.01) levels; increase glutathione peroxidase activity (P<0.001); upregulate cell mitochondrial membrane potential level (P<0.001); regulate apoptosis-related protei
ISSN:1002-0306
DOI:10.13386/j.issn1002-0306.2021120012