Effect of Zataria multiflora Boiss. Essential Oil on Growth Curve and Shigatoxin 2 Production of Enterohemorrhagic Escherichia Coli O157:H7

• Published in: Journal of medicinal plants (Online) Vol. 12; no. 48; pp. 62 - 71
• Main Authors: Ataee M, Akhondzadeh Basti A, Zahraei Salehi T, Hosseini H, Gandomi Nasrabadi H, Noori N, Khanjari A, Taheri Mirghaed A, Mohammad khan F, Faghih Fard P
• Format: Journal Article
• Language: English; Persian
• Published: Institue of Medicinal Plants, ACECR 01.12.2013
• Subjects: escherichia coli o157:h7; shigatoxin; zataria multiflora boiss
• ISSN: 2717-204X; 2717-2058

Background: Zataria multiflora Boiss. is one of the Iranian traditional spices and it has antimicrobial effect on the pathogenic bacteria which are agents for some current foodborne intoxications. Objectives: The present study was conducted to evaluate the antimicrobial activity of Zataria multiflora Boiss. Essential oil on Escherichia coli O157:H7. Methods: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Zataria multiflora Boiss. essential oil (EO) for Escherichia coli O157:H7 at 35°C, the effect of subinhibitory concentrations of EO on growth curve of bacteria up to 24 hours at 25 and 35°C and also production of shigatoxin 2 (Stx2), at 35°C has been determined. Results: MIC and MBC of EO which have been evaluated were 0.04 and 0.06%, respectively the effect of subinhibitory concentrations of EO on bacterial growth curve during 24 hours has been determined by spectrophotometer device at 25 and 35°C and also production of shigatoxin 2 (Stx2), at 35°C has been determined. Subihibitory concentrations of EO, significantly, decreased the production of Stx2 at 35°C in a dose dependent manner. Conclusion: The results showed that Zataria multiflora Boiss. essential oil had inhibitory effect on Escherichia coli O157:H7and it can be used as a natural preservative in food industry.