Metals Accumulation and Leaf Surface Anatomy of Murdannia spectabilis Growing in Zn/Cd Contaminated Soil

• Published in: EnvironmentAsia Vol. 6; no. 2; pp. 71 - 82
• Main Authors: Ladawan Rattanapolsan, Woranan Nakbanpote, Piyaporn Saensouk
• Format: Journal Article
• Language: English
• Published: Thai Society of Higher Eduction Institutes on Environment 01.07.2013
• Subjects: anatomy; cadmium; Murdannia; phytoremediation; zinc
• ISSN: 1906-1714

Murdannia spectabilis (Kurz) Faden was identified as a Zn/Cd hyperaccumulative plant. Leaf surface anatomy of the plant growing in non-contaminated soil (control) and Zn/Cd contaminated soil,was studied and compared by a light microscopy and scanning electron microscopy combined with Energy-dispersive X-ray spectroscopy(SEM/EDS). The similarities were reticulate cuticle on epidermises, uniform polygonal cell, stomatal arrangement in six surrounding subsidiary cells, and submarginal sclerenchyma. The dissimilarities were uniserate trichomes spreading on both adaxial and abaxial epidermis of the plants growing in non-contaminated soil, whereas the uniserate trichomes were only on the submarginal-adaxial epidermis of the control plants. The trichomes on leaves of the plants growing in non-contaminated soil were found to have both uniseriate non-glandular and uniseriate glandular trichomes;whereas, leaves of the plants growing in the contaminated soil were merely non-glandular trichomes. The different shape and location of trichomes, the number of stomata and trichome indicated the effect of Zn and Cd on M. spectabilis. The higher percentages of Zn and Cd in the vascular bundle than in the cross section and epidermis areas showed both solutes could move along each route, with diffusion through the symplast and apoplast. The increase of Ca in M. spectabilis growing in Zn/Cd contaminated soil corresponded to the Zn and Cd distributed in the leaves. Zn K-edge and S K-edge XANES spectra proposed that Zn2+ ions were accumulated and/or adsorbed on the epidermis of the tuber, and then absorbed into the root and transport to the xylem. The double peaks of Zn-cysteine in the leaf samples proposed the metal sequestration was by sulphur proteins.