Antibacterial Activities of Selenium Nanoparticles Against Multidrug Resistant Staphylococcus aureus and Escherichia coli Isolated from Mastitic Milk

• Published in: Journal of advanced veterinary research Vol. 12; no. 6
• Main Authors: Mona M. El-Azzouny, Sabah I. Khater, Sara H. Adli, Sally H. Abou-Khadra
• Format: Journal Article
• Language: English
• Published: Assiut University 06.11.2022
• ISSN: 2090-6269; 2090-6277

Bovine mastitis is one of the most important diseases affecting dairy cows and subsequently milk production. There are several causes leading to the onset of mastitis in dairy cows, bacterial diseases are among the most important etiological agents. Therefore, this study was conducted to investigate the antibacterial activity of selenium nanoparticles against two major organisms causing mastitis in cows. Bacteriological examination of milk samples in the present study revealed that 25(40.32%) and, 27(43.54%) were positive for Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), respectively. Among the examined samples 15(24.19%) had mixed E. coli and S. aureus infection. The antimicrobial susceptibility patterns showed that most isolates of S. aureus and E. coli were multidrug resistant, on the other hand they were sensitive to ciprofloxacin and gentamycin.  Broth microdilution method was used for determination of the minimum inhibitory concentrations (MIC) of selenium nanoparticles against S. aureus and E. coli isolates.  The MIC values against S. aureus isolates ranged from 15.62 to 31.25µg/ml, but in E. coli isolates ranged between 31.25 and 62.5 µg/ml. This in vitro result clearly indicates that the selenium nanoparticle have a good antibacterial activity against multidrug resistant bacteria causing mastitis.