Study of the Transcript and Protein Expression of Poliovirus Receptor (CD155 Protein) on Colorectal Cancer Cell Line

• Published in: Majallah-i dānishkadah-i pizishkī-i Iṣfahān. (Online) Vol. 35; no. 427; pp. 453 - 462
• Main Authors: Sareh Zhand, Seyed Masoud Hoseini, Alijan Tabarraei, Mohsen Saeedi, Abdolvahab Moradi
• Format: Journal Article
• Language: Persian
• Published: Isfahan University of Medical Sciences 01.06.2017
• Subjects: CD155 protein; Colorectal cancer; Flow cytometry; Gene expression; Real-Time polymerase chain reaction
• ISSN: 1027-7595; 1735-854X

Background: Poliovirus receptor (CD155 protein or PVR) expressed on many types of cells and exerts diverse functions. Several studies have demonstrated that changes in CD155 expression in cancer cell lines affect metastasis, proliferation, and migration. The purpose of the present study was to investigate the transcript and protein expression of CD155 in human colon adenocarcinoma cell lines in comparison to normal fetal human colon (FHC) cells. Methods: The CD155 expression levels in a human adenocarcinoma cell line and normal colon cell line were evaluated using the quantitative real-time polymerase chain reaction (PCR) and flow cytometry. All statistical analyses were performed using SPSS software at the statistical significance level of P < 0.050. Findings: Real-time polymerase chain reaction indicated that CD155 significantly overexpressed in human adenocarcinoma cell line significantly more than normal fetal cells (P < 0.001). Flow cytometry showed that protein was strongly expressed in cancer cell line and SW480 cell line showed the highest CD155 protein expression level of 98.0%, whereas this protein expression was 1.3% in human normal colon cell line (P < 0.001). Conclusion: Collectively, these data indicate that CD155 expression is frequently elevated in cancer cell line. The preferential expression of CD155 on cancer cell line rather than on normal cell line suggests that CD155 could be targeted for future poliovirus virotherapy.