The green impact: bacterioplankton response towards a phytoplankton spring bloom in the southern North Sea assessed by comparative metagenomic and metatranscriptomic approaches

• Published in: Frontiers in microbiology Vol. 6
• Main Authors: Bernd eWemheuer, Franziska eWemheuer, Jacqueline eHollensteiner, Frauke-Dorothee eMeyer, Sonja eVoget, Rolf eDaniel
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 01.08.2015
• Subjects: Algal Bloom; bacterioplankton; Metagenomics; metatranscriptomics; Planktomarina temperata; SAR92
• ISSN: 1664-302X
• DOI: 10.3389/fmicb.2015.00805

Phytoplankton blooms exhibit a severe impact on bacterioplankton communities as they change nutrient availabilities and other environmental factors. In the current study, the response of a bacterioplankton community to a Phaeocystis globosa spring bloom was investigated in the southern North Sea. For this purpose, water samples were taken inside and reference samples outside of an algal spring bloom. Structural changes of the bacterioplankton community were assessed by amplicon-based analysis of 16S rRNA genes and transcripts generated from environmental DNA and RNA, respectively. Several marine groups responded to bloom presence. The abundance of the Roseobacter RCA cluster and the SAR92 clade significantly increased in bloom presence in the total and active fraction of the bacterial community. Functional changes were investigated by direct sequencing of environmental DNA and mRNA. The corresponding datasets comprised more than 500 million sequences across all samples. Metatranscriptomic data sets were mapped on representative genomes of abundant marine groups present in the samples and on assembled metagenomic and metatranscriptomic datasets. Differences in gene expression profiles between non-bloom and bloom samples were recorded. The genome-wide gene expression level of Planktomarina temperata, an abundant member of the Roseobacter RCA cluster, was higher inside the bloom. Genes that were differently expressed