Effect and mechanism of reactive oxygen species-responsive nanoparticles on the regulation of human gingival fibroblast function and inflammation induced by lipopolysaccharide

• Published in: Kou qiang ji bing fang zhi = Journal of prevention and treatment for stomatological diseases Vol. 32; no. 4; pp. 257 - 265
• Main Author: QIU Xinyi, SONG Lutong, REN Shuangshuang, MIAO Leiying
• Format: Journal Article
• Language: Chinese
• Published: Editorial Department of Journal of Prevention and Treatment for Stomatological Diseases 20.04.2024
• Subjects: human gingival fibroblasts|reactive oxygen species|porphyromonas gingivalis|lipopolysaccharide|inflammation|cell migration|nanoparticles|collagen
• ISSN: 2096-1456
• DOI: 10.12016/j.issn.2096-1456.2024.04.003

Objective To investigate the effects of PssL-NAC reactive oxygen species (ROS)-responsive nanoparticles on intracellular ROS production, inflammatory factor levels, collagen production, cell function and Toll-like receptor 4 (TLR4), NF-κB nuclear factor-κB (p65) pathway protein expression in human gingival fibroblasts (HGFs) induced by Porphyromonas gingivalis-lipopolysaccharide (P.g-LPS). Methods This study was reviewed and approved by the ethics committee. PssL-NAC microspheres containing oil soluble antioxidant N-acetylcysteine (NAC) were obtained by connecting the hydrophobic end of polycaprolactone (PCL) and the hydrophilic end of polyethylene glycol (PEG) via thioketal (TK) bonds in response to ROS, and self loading in the aqueous and oil phases. After preparation of the PssL-NAC microspheres and aqueous NAC solution, successful synthesis of the nanoparticles was verified by transmission electron microscopy. Then, HGFs were exposed to P.g-LPS (0, 5, or 10 μg/mL), P.g-LPS (0, 5, or 10 μg/mL)+NAC, and P.g