Synergistic Inactivation Effects and Mechanisms of Plasma-Activated Water Combined with Phenyllactic Acid against Escherichia coli O157:H7

The present study aimed to elucidate the antibacterial effect and underlying mechanisms of plasma-activated water (PAW) combined with phenyllactic acid (PLA) against Escherichia coli O157:H7. The inactivation effects of PAW and PLA on E. coli O157:H7 and their effect on cell morphology, cell membran...

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Bibliographic Details
Published inShipin gongye ke-ji Vol. 43; no. 14; pp. 138 - 143
Main Authors Dianbo ZHAO, Shaodan WANG, Kaixi ZHENG, Qisen XIANG, Bohua WANG, Qifan ZHANG
Format Journal Article
LanguageChinese
Published The editorial department of Science and Technology of Food Industry 01.07.2022
Subjects
escherichia coli o157:h7
mechanisms
phenyllactic acid
plasma-activated water
sterilizing effect
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Summary:The present study aimed to elucidate the antibacterial effect and underlying mechanisms of plasma-activated water (PAW) combined with phenyllactic acid (PLA) against Escherichia coli O157:H7. The inactivation effects of PAW and PLA on E. coli O157:H7 and their effect on cell morphology, cell membrane integrity and intracellular reactive oxygen species (ROS) level were investigated by plate counting, scanning electron microscopy and fluorescence staining. The results showed that the population of E. coli O157:H7 did not change significantly after PLA treatment at 0.125～1.0 mg/mL for 8 min (P>0.05). E. coli O157:H7 decreased by 5.65 lg CFU/mL after PAW treatment combined with 1.0 mg/mL of PLA for 8 min, which was significantly higher than the 1.06 lg CFU/mL of PAW alone (P<0.05). As shown by the scanning electron microscope images, obvious changes in the morphology of E. coli O157: H7 cells were observed after PAW treatment combined with PLA. After the co-treatment of PAW and PLA (1.0 mg/mL) for 8 min, the extr
ISSN:1002-0306
DOI:10.13386/j.issn1002-0306.2021110116