In vitro growth of sweet potato fed with potassium phosphite/Crescimento in vitro de batata-doce nutrida com fosfito de potassio

The main objective of this work was to verify the effect of the substitution of phosphate by its related compound phosphite on the in vitro growth of sweet potato nodal segments. Segments from a previous culture were incubated in MS media with the following combinations of phosphate:phosphite: Contr...

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Published inActa scientiarum. Agronomy Vol. 34; no. 1; pp. 85 - 91
Main Authors Hirosse, Edison Hitoshi, Creste, Jose Eduardo, Custodio, Ceci Castilho, Machado-Neto, Nelson Barbosa
Format Journal Article
LanguageSpanish
English
Published Universidade Estadual de Maringa 01.01.2012
Eduem (Editora da Universidade Estadual de Maringá)
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Summary:The main objective of this work was to verify the effect of the substitution of phosphate by its related compound phosphite on the in vitro growth of sweet potato nodal segments. Segments from a previous culture were incubated in MS media with the following combinations of phosphate:phosphite: Control (100 / 0%); T1 (87.5 / 12.5%); T2 (75 / 25%); T3 (50 / 50%) and T4 (0 / 100%) in a 5 x 5 factorial arrangement, with five treatments (Control, T1, T2, T3 and T4) and five evaluation periods (7, 14, 21, 28 and 35 days). The design was completely random, with three replicates of two explants per period. The evaluations were made weekly by counting the number of shoots and the length and dry weight of shoots and roots. Increasing the phosphite concentration decreased all attributes measured, including growth velocity, length and dry weight. These results indicate that phosphite cannot be used as substituted of phosphate in sweet potato tissue cultures. Keywords: Ipomoea batatas, nutrition, plantlet, shoot, root. O objetivo deste trabalho foi verificar o efeito da substituicao do fosfato pelo composto derivado fosfito no crescimento in vitro de segmentos nodais de batata doce. Os segmentos obtidos de culturas previas foram incubados em meio MS com as seguintes combinacOes de fosfato/fosfito: Controle (100 / 0%); T1 (87.5 /12.5%); T2 (75 / 25%); T3 (50 / 50%) e T4 (0 / 100%) respectivamente, em arranjo fatorial de 5x5, com cinco tratamentos (Controle, T1, T2, T3 e T4) e cinco periodos de avaliacao (7, 14, 21, 28 e 35 dias). O desenho experimental foi completamente casualizado com tres repeticOes com dois explantes por periodo. As avaliacOes foram feitas semanalmente por contagem do numero de brotos, comprimento e peso seco de parte aerea de brotos e raizes. O aumento da dose de fosfito diminuiu todos os atributos analisados, como velocidade de crescimento, comprimento e peso seco, significando que o fosfito nao pode ser utilizado em substituicao ao fosfato na cultura de tecidos de batata doce. Palavras-chave: Ipomoea batatas, nutricao, plantlete, broto, raiz.
ISSN:1679-9275
1807-8621
DOI:10.4025/actasciagron.v34i1.10810