Liquid exfoliation of 2D MoS 2 nanosheets and their utilization as a label-free electrochemical immunoassay for subclinical ketosis
We report the step-by-step fabrication of a 2D MoS nanostructure-based disposable electrochemical immunosensor to detect β-hydroxybutyrate (βHBA), a novel subclinical ketosis biomarker. The MoS nanosheets were exfoliated in the liquid phase by ultrasonication, and then followed by deposition on gold...
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| Published in | Nanoscale Vol. 9; no. 30; pp. 10886 - 10896 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
England
03.08.2017
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| Subjects | |
| Online Access | Get full text |
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| Abstract | We report the step-by-step fabrication of a 2D MoS
nanostructure-based disposable electrochemical immunosensor to detect β-hydroxybutyrate (βHBA), a novel subclinical ketosis biomarker. The MoS
nanosheets were exfoliated in the liquid phase by ultrasonication, and then followed by deposition on gold colloid modified screen-printed electrodes (Au-SPE). The MoS
-modified electrodes were thoroughly characterized by physical, electrochemical as well as spectroscopic techniques, and the obtained results indicate the successful and irreversible electrodeposition of MoS
nanosheets. These MoS
-modified disposable electrodes were subsequently bioconjugated with anti-βHBA antibodies and then employed for the label-free immunosensing of the βHBA biomarker to detect subclinical ketosis. A simple electrochemical Differential Pulse Voltammetry (DPV) technique based immunodetection was realized for the sensing of varying concentrations of βHBA antigen. The bioassay demonstrated remarkable sensitivity and specificity having a dynamic linear response range of 0.7 mM to 10 mM for βHBA in standard antigen solutions, spiked serum, blood, milk and clinical samples, with linear results being obtained with R
∼ 0.9923. The sensor electrodes were also studied with other relative interferents to investigate cross reactivity and non-specificity. These electrodes showed a linear, specific, reproducible and stable response towards the βHBA antigen over a wide range of concentrations. |
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| AbstractList | We report the step-by-step fabrication of a 2D MoS
2
nanostructure-based disposable electrochemical immunosensor to detect β-hydroxybutyrate (βHBA), a novel subclinical ketosis biomarker. The MoS
2
nanosheets were exfoliated in the liquid phase by ultrasonication, and then followed by deposition on gold colloid modified screen-printed electrodes (Au-SPE). The MoS
2
-modified electrodes were thoroughly characterized by physical, electrochemical as well as spectroscopic techniques, and the obtained results indicate the successful and irreversible electrodeposition of MoS
2
nanosheets. These MoS
2
-modified disposable electrodes were subsequently bioconjugated with anti-βHBA antibodies and then employed for the label-free immunosensing of the βHBA biomarker to detect subclinical ketosis. A simple electrochemical Differential Pulse Voltammetry (DPV) technique based immunodetection was realized for the sensing of varying concentrations of βHBA antigen. The bioassay demonstrated remarkable sensitivity and specificity having a dynamic linear response range of 0.7 mM to 10 mM for βHBA in standard antigen solutions, spiked serum, blood, milk and clinical samples, with linear results being obtained with
R
2
∼ 0.9923. The sensor electrodes were also studied with other relative interferents to investigate cross reactivity and non-specificity. These electrodes showed a linear, specific, reproducible and stable response towards the βHBA antigen over a wide range of concentrations. We report the step-by-step fabrication of a 2D MoS nanostructure-based disposable electrochemical immunosensor to detect β-hydroxybutyrate (βHBA), a novel subclinical ketosis biomarker. The MoS nanosheets were exfoliated in the liquid phase by ultrasonication, and then followed by deposition on gold colloid modified screen-printed electrodes (Au-SPE). The MoS -modified electrodes were thoroughly characterized by physical, electrochemical as well as spectroscopic techniques, and the obtained results indicate the successful and irreversible electrodeposition of MoS nanosheets. These MoS -modified disposable electrodes were subsequently bioconjugated with anti-βHBA antibodies and then employed for the label-free immunosensing of the βHBA biomarker to detect subclinical ketosis. A simple electrochemical Differential Pulse Voltammetry (DPV) technique based immunodetection was realized for the sensing of varying concentrations of βHBA antigen. The bioassay demonstrated remarkable sensitivity and specificity having a dynamic linear response range of 0.7 mM to 10 mM for βHBA in standard antigen solutions, spiked serum, blood, milk and clinical samples, with linear results being obtained with R ∼ 0.9923. The sensor electrodes were also studied with other relative interferents to investigate cross reactivity and non-specificity. These electrodes showed a linear, specific, reproducible and stable response towards the βHBA antigen over a wide range of concentrations. |
| Author | Tuteja, Satish K Neethirajan, Suresh Duffield, Todd |
| Author_xml | – sequence: 1 givenname: Satish K surname: Tuteja fullname: Tuteja, Satish K email: sneethir@uoguelph.ca organization: BioNano Laboratory, School of Engineering, University of Guelph, Guelph, ON N1G 2W1, Canada. sneethir@uoguelph.ca – sequence: 2 givenname: Todd surname: Duffield fullname: Duffield, Todd – sequence: 3 givenname: Suresh surname: Neethirajan fullname: Neethirajan, Suresh |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28731116$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1021/cm502915f 10.1038/ncomms3995 10.1021/nl4042356 10.3168/jds.2009-2277 10.1016/j.trac.2014.05.009 10.1021/acs.nanolett.5b03218 10.1021/acsami.6b03079 10.1021/nl903868w 10.1038/srep07352 10.1039/C6NR06836G 10.1039/C4CP01598C 10.1038/nnano.2010.279 10.1021/acs.jpcc.6b01838 10.1038/nchem.1589 10.1002/smll.201302081 10.1021/am503524e 10.1007/s00604-016-1803-x 10.1016/j.apmt.2016.09.012 10.1002/adma.201503163 10.1021/am4034476 10.1021/am509096x 10.1016/j.aca.2016.08.010 10.1016/j.bios.2016.07.052 10.1016/j.bios.2015.05.008 10.1021/nl4014748 10.1021/ar500303m 10.1021/nn500064s 10.1021/nl3043079 10.3168/jds.2008-1795 10.1002/ange.201000009 10.1103/PhysRevLett.105.136805 10.1038/srep43599 10.1038/nchem.2108 10.1021/nn400026u 10.1103/PhysRevB.84.205325 10.1071/AN14503 10.1016/j.snb.2016.01.028 10.1371/journal.pone.0080316 10.1038/srep01839 10.1021/ac402114c 10.1002/cnma.201600363 10.3168/jds.2011-4463 10.1021/nn5009148 10.1021/nl201874w 10.1039/C5NR02253C 10.1039/C5NR06121K 10.1021/nn400280c 10.1073/pnas.0502848102 10.1021/nn4041987 10.1038/nnano.2014.176 10.1186/s12951-015-0076-6 10.1039/C5NR06144J 10.1016/j.mattod.2015.08.019 10.1039/C5NR02224J 10.1002/adfm.201500891 10.1016/j.bios.2016.04.058 10.1021/nn1003937 10.1002/adfm.201102111 |
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| References | Shi (C7NR04307D-(cit52)/*[position()=1]) 2013; 3 Presolski (C7NR04307D-(cit31)/*[position()=1]) 2016; 19 Lee (C7NR04307D-(cit61)/*[position()=1]) 2014; 4 Nguyen (C7NR04307D-(cit45)/*[position()=1]) 2015; 27 Kukkar (C7NR04307D-(cit59)/*[position()=1]) 2016; 939 Novoselov (C7NR04307D-(cit7)/*[position()=1]) 2005; 102 Splendiani (C7NR04307D-(cit10)/*[position()=1]) 2010; 10 Lee (C7NR04307D-(cit25)/*[position()=1]) 2014; 4 Zhang (C7NR04307D-(cit17)/*[position()=1]) 2015; 7 Wang (C7NR04307D-(cit1)/*[position()=1]) 2014; 10 Ramakrishna Matte (C7NR04307D-(cit4)/*[position()=1]) 2010; 122 McArt (C7NR04307D-(cit38)/*[position()=1]) 2011; 94 Kalantar-zadeh (C7NR04307D-(cit62)/*[position()=1]) 2016; 5 Wang (C7NR04307D-(cit23)/*[position()=1]) 2013; 85 Tuteja (C7NR04307D-(cit43)/*[position()=1]) 2014; 6 Sordillo (C7NR04307D-(cit36)/*[position()=1]) 2014; 9 Jiang (C7NR04307D-(cit8)/*[position()=1]) 2014; 9 Radisavljevic (C7NR04307D-(cit22)/*[position()=1]) 2011; 6 Weng (C7NR04307D-(cit40)/*[position()=1]) 2015; 72 Jariwala (C7NR04307D-(cit24)/*[position()=1]) 2014; 8 Mak (C7NR04307D-(cit11)/*[position()=1]) 2010; 105 Kukkar (C7NR04307D-(cit26)/*[position()=1]) 2016; 8 Iwersen (C7NR04307D-(cit37)/*[position()=1]) 2009; 92 Ramasubramaniam (C7NR04307D-(cit12)/*[position()=1]) 2011; 84 Nguyen (C7NR04307D-(cit54)/*[position()=1]) 2016; 120 Conley (C7NR04307D-(cit9)/*[position()=1]) 2013; 13 Nguyen (C7NR04307D-(cit28)/*[position()=1]) 2015; 27 Late (C7NR04307D-(cit21)/*[position()=1]) 2013; 7 Chhowalla (C7NR04307D-(cit5)/*[position()=1]) 2013; 5 Zheng (C7NR04307D-(cit50)/*[position()=1]) 2014; 5 Lee (C7NR04307D-(cit46)/*[position()=1]) 2010; 4 Singh (C7NR04307D-(cit57)/*[position()=1]) 2016; 183 Wang (C7NR04307D-(cit60)/*[position()=1]) 2014; 10 Weng (C7NR04307D-(cit41)/*[position()=1]) 2015; 13 Mehta (C7NR04307D-(cit48)/*[position()=1]) 2016; 83 Ou (C7NR04307D-(cit20)/*[position()=1]) 2014; 14 Van Le (C7NR04307D-(cit53)/*[position()=1]) 2014; 16 Nguyen (C7NR04307D-(cit33)/*[position()=1]) 2016 Wang (C7NR04307D-(cit56)/*[position()=1]) 2013; 7 Lv (C7NR04307D-(cit27)/*[position()=1]) 2016; 8 Perkins (C7NR04307D-(cit15)/*[position()=1]) 2013; 13 Veerapandian (C7NR04307D-(cit42)/*[position()=1]) 2016; 228 Sarkar (C7NR04307D-(cit19)/*[position()=1]) 2014; 8 Scharf (C7NR04307D-(cit30)/*[position()=1]) 2013; 5 Yuan (C7NR04307D-(cit35)/*[position()=1]) 2013; 8 Pumera (C7NR04307D-(cit2)/*[position()=1]) 2014; 61 Kukkar (C7NR04307D-(cit29)/*[position()=1]) 2016; 939 Huang (C7NR04307D-(cit3)/*[position()=1]) 2015; 7 Eda (C7NR04307D-(cit51)/*[position()=1]) 2011; 11 Butler (C7NR04307D-(cit6)/*[position()=1]) 2013; 7 Voiry (C7NR04307D-(cit34)/*[position()=1]) 2014; 7 Zhao (C7NR04307D-(cit14)/*[position()=1]) 2015; 48 Kalantar-zadeh (C7NR04307D-(cit18)/*[position()=1]) 2015; 25 Han (C7NR04307D-(cit58)/*[position()=1]) 2017; 7 Rao (C7NR04307D-(cit16)/*[position()=1]) 2015; 7 Satheeshkumar (C7NR04307D-(cit32)/*[position()=1]) 2017; 3 Ospina (C7NR04307D-(cit39)/*[position()=1]) 2010; 93 Chu (C7NR04307D-(cit13)/*[position()=1]) 2015; 15 Zhang (C7NR04307D-(cit44)/*[position()=1]) 2015; 7 Tuteja (C7NR04307D-(cit49)/*[position()=1]) 2016; 86 Jia (C7NR04307D-(cit55)/*[position()=1]) 2015; 7 Li (C7NR04307D-(cit47)/*[position()=1]) 2012; 22 |
| References_xml | – volume: 27 start-page: 53 year: 2015 ident: C7NR04307D-(cit45)/*[position()=1] publication-title: Chem. Mater. doi: 10.1021/cm502915f contributor: fullname: Nguyen – volume: 5 start-page: 2995 year: 2014 ident: C7NR04307D-(cit50)/*[position()=1] publication-title: Nat. Commun. doi: 10.1038/ncomms3995 contributor: fullname: Zheng – volume: 14 start-page: 857 year: 2014 ident: C7NR04307D-(cit20)/*[position()=1] publication-title: Nano Lett. doi: 10.1021/nl4042356 contributor: fullname: Ou – volume: 93 start-page: 546 year: 2010 ident: C7NR04307D-(cit39)/*[position()=1] publication-title: J. Dairy Sci. doi: 10.3168/jds.2009-2277 contributor: fullname: Ospina – volume: 61 start-page: 49 year: 2014 ident: C7NR04307D-(cit2)/*[position()=1] publication-title: TrAC, Trends Anal. Chem. doi: 10.1016/j.trac.2014.05.009 contributor: fullname: Pumera – volume: 15 start-page: 8000 year: 2015 ident: C7NR04307D-(cit13)/*[position()=1] publication-title: Nano Lett. doi: 10.1021/acs.nanolett.5b03218 contributor: fullname: Chu – volume: 8 start-page: 16555 year: 2016 ident: C7NR04307D-(cit26)/*[position()=1] publication-title: ACS Appl. Mater. Interfaces doi: 10.1021/acsami.6b03079 contributor: fullname: Kukkar – volume: 10 start-page: 1271 year: 2010 ident: C7NR04307D-(cit10)/*[position()=1] publication-title: Nano Lett. doi: 10.1021/nl903868w contributor: fullname: Splendiani – volume: 4 start-page: 7352 year: 2014 ident: C7NR04307D-(cit61)/*[position()=1] publication-title: Sci. Rep. doi: 10.1038/srep07352 contributor: fullname: Lee – volume: 8 start-page: 18250 year: 2016 ident: C7NR04307D-(cit27)/*[position()=1] publication-title: Nanoscale doi: 10.1039/C6NR06836G contributor: fullname: Lv – volume: 16 start-page: 13123 year: 2014 ident: C7NR04307D-(cit53)/*[position()=1] publication-title: Phys. Chem. Chem. Phys. doi: 10.1039/C4CP01598C contributor: fullname: Van Le – volume: 6 start-page: 147 year: 2011 ident: C7NR04307D-(cit22)/*[position()=1] publication-title: Nat. Nanotechnol. doi: 10.1038/nnano.2010.279 contributor: fullname: Radisavljevic – volume: 120 start-page: 10078 year: 2016 ident: C7NR04307D-(cit54)/*[position()=1] publication-title: J. Phys. Chem. C doi: 10.1021/acs.jpcc.6b01838 contributor: fullname: Nguyen – volume: 5 start-page: 263 year: 2013 ident: C7NR04307D-(cit5)/*[position()=1] publication-title: Nat. Chem. doi: 10.1038/nchem.1589 contributor: fullname: Chhowalla – volume: 10 start-page: 1101 year: 2014 ident: C7NR04307D-(cit1)/*[position()=1] publication-title: Small doi: 10.1002/smll.201302081 contributor: fullname: Wang – volume: 6 start-page: 14767 year: 2014 ident: C7NR04307D-(cit43)/*[position()=1] publication-title: ACS Appl. Mater. Interfaces doi: 10.1021/am503524e contributor: fullname: Tuteja – volume: 183 start-page: 1729 year: 2016 ident: C7NR04307D-(cit57)/*[position()=1] publication-title: Microchim. Acta doi: 10.1007/s00604-016-1803-x contributor: fullname: Singh – volume: 5 start-page: 73 year: 2016 ident: C7NR04307D-(cit62)/*[position()=1] publication-title: Appl. Mater. Today doi: 10.1016/j.apmt.2016.09.012 contributor: fullname: Kalantar-zadeh – volume: 27 start-page: 6225 year: 2015 ident: C7NR04307D-(cit28)/*[position()=1] publication-title: Adv. Mater. doi: 10.1002/adma.201503163 contributor: fullname: Nguyen – volume: 5 start-page: 11762 year: 2013 ident: C7NR04307D-(cit30)/*[position()=1] publication-title: ACS Appl. Mater. Interfaces doi: 10.1021/am4034476 contributor: fullname: Scharf – volume: 7 start-page: 7809 year: 2015 ident: C7NR04307D-(cit16)/*[position()=1] publication-title: ACS Appl. Mater. Interfaces doi: 10.1021/am509096x contributor: fullname: Rao – volume: 939 start-page: 101 year: 2016 ident: C7NR04307D-(cit59)/*[position()=1] publication-title: Anal. Chim. Acta doi: 10.1016/j.aca.2016.08.010 contributor: fullname: Kukkar – volume: 86 start-page: 548 year: 2016 ident: C7NR04307D-(cit49)/*[position()=1] publication-title: Biosens. Bioelectron. doi: 10.1016/j.bios.2016.07.052 contributor: fullname: Tuteja – volume: 72 start-page: 140 year: 2015 ident: C7NR04307D-(cit40)/*[position()=1] publication-title: Biosens. Bioelectron. doi: 10.1016/j.bios.2015.05.008 contributor: fullname: Weng – volume: 13 start-page: 3626 year: 2013 ident: C7NR04307D-(cit9)/*[position()=1] publication-title: Nano Lett. doi: 10.1021/nl4014748 contributor: fullname: Conley – volume: 48 start-page: 91 year: 2015 ident: C7NR04307D-(cit14)/*[position()=1] publication-title: Acc. Chem. Res. doi: 10.1021/ar500303m contributor: fullname: Zhao – volume: 8 start-page: 1102 year: 2014 ident: C7NR04307D-(cit24)/*[position()=1] publication-title: ACS Nano doi: 10.1021/nn500064s contributor: fullname: Jariwala – volume: 13 start-page: 668 year: 2013 ident: C7NR04307D-(cit15)/*[position()=1] publication-title: Nano Lett. doi: 10.1021/nl3043079 contributor: fullname: Perkins – volume: 92 start-page: 2618 year: 2009 ident: C7NR04307D-(cit37)/*[position()=1] publication-title: J. Dairy Sci. doi: 10.3168/jds.2008-1795 contributor: fullname: Iwersen – volume: 122 start-page: 4153 year: 2010 ident: C7NR04307D-(cit4)/*[position()=1] publication-title: Angew. Chem. doi: 10.1002/ange.201000009 contributor: fullname: Ramakrishna Matte – volume: 105 start-page: 136805 year: 2010 ident: C7NR04307D-(cit11)/*[position()=1] publication-title: Phys. Rev. Lett. doi: 10.1103/PhysRevLett.105.136805 contributor: fullname: Mak – volume: 10 start-page: 1101 year: 2014 ident: C7NR04307D-(cit60)/*[position()=1] publication-title: Small doi: 10.1002/smll.201302081 contributor: fullname: Wang – volume-title: Current Protocols in Chemical Biology year: 2016 ident: C7NR04307D-(cit33)/*[position()=1] contributor: fullname: Nguyen – volume: 7 start-page: 43599 year: 2017 ident: C7NR04307D-(cit58)/*[position()=1] publication-title: Sci. Rep. doi: 10.1038/srep43599 contributor: fullname: Han – volume: 7 start-page: 45 year: 2014 ident: C7NR04307D-(cit34)/*[position()=1] publication-title: Nat. Chem. doi: 10.1038/nchem.2108 contributor: fullname: Voiry – volume: 7 start-page: 4879 year: 2013 ident: C7NR04307D-(cit21)/*[position()=1] publication-title: ACS Nano doi: 10.1021/nn400026u contributor: fullname: Late – volume: 84 start-page: 205325 year: 2011 ident: C7NR04307D-(cit12)/*[position()=1] publication-title: Phys. Rev. B: Condens. Matter doi: 10.1103/PhysRevB.84.205325 contributor: fullname: Ramasubramaniam – volume: 9 start-page: 1204 year: 2014 ident: C7NR04307D-(cit36)/*[position()=1] publication-title: Anim. Prod. Sci. doi: 10.1071/AN14503 contributor: fullname: Sordillo – volume: 228 start-page: 180 year: 2016 ident: C7NR04307D-(cit42)/*[position()=1] publication-title: Sens. Actuators, B doi: 10.1016/j.snb.2016.01.028 contributor: fullname: Veerapandian – volume: 8 start-page: e80316 year: 2013 ident: C7NR04307D-(cit35)/*[position()=1] publication-title: PLoS One doi: 10.1371/journal.pone.0080316 contributor: fullname: Yuan – volume: 3 start-page: 1839 year: 2013 ident: C7NR04307D-(cit52)/*[position()=1] publication-title: Sci. Rep. doi: 10.1038/srep01839 contributor: fullname: Shi – volume: 85 start-page: 10289 year: 2013 ident: C7NR04307D-(cit23)/*[position()=1] publication-title: Anal. Chem. doi: 10.1021/ac402114c contributor: fullname: Wang – volume: 3 start-page: 172 year: 2017 ident: C7NR04307D-(cit32)/*[position()=1] publication-title: ChemNanoMat doi: 10.1002/cnma.201600363 contributor: fullname: Satheeshkumar – volume: 94 start-page: 6011 year: 2011 ident: C7NR04307D-(cit38)/*[position()=1] publication-title: J. Dairy Sci. doi: 10.3168/jds.2011-4463 contributor: fullname: McArt – volume: 8 start-page: 3992 year: 2014 ident: C7NR04307D-(cit19)/*[position()=1] publication-title: ACS Nano doi: 10.1021/nn5009148 contributor: fullname: Sarkar – volume: 11 start-page: 5111 year: 2011 ident: C7NR04307D-(cit51)/*[position()=1] publication-title: Nano Lett. doi: 10.1021/nl201874w contributor: fullname: Eda – volume: 7 start-page: 10210 year: 2015 ident: C7NR04307D-(cit44)/*[position()=1] publication-title: Nanoscale doi: 10.1039/C5NR02253C contributor: fullname: Zhang – volume: 7 start-page: 18364 year: 2015 ident: C7NR04307D-(cit17)/*[position()=1] publication-title: Nanoscale doi: 10.1039/C5NR06121K contributor: fullname: Zhang – volume: 7 start-page: 2898 year: 2013 ident: C7NR04307D-(cit6)/*[position()=1] publication-title: ACS Nano doi: 10.1021/nn400280c contributor: fullname: Butler – volume: 4 start-page: 7352 year: 2014 ident: C7NR04307D-(cit25)/*[position()=1] publication-title: Sci. Rep. doi: 10.1038/srep07352 contributor: fullname: Lee – volume: 102 start-page: 10451 year: 2005 ident: C7NR04307D-(cit7)/*[position()=1] publication-title: Proc. Natl. Acad. Sci. U. S. A. doi: 10.1073/pnas.0502848102 contributor: fullname: Novoselov – volume: 7 start-page: 10083 year: 2013 ident: C7NR04307D-(cit56)/*[position()=1] publication-title: ACS Nano doi: 10.1021/nn4041987 contributor: fullname: Wang – volume: 9 start-page: 825 year: 2014 ident: C7NR04307D-(cit8)/*[position()=1] publication-title: Nat. Nanotechnol. doi: 10.1038/nnano.2014.176 contributor: fullname: Jiang – volume: 13 start-page: 13 year: 2015 ident: C7NR04307D-(cit41)/*[position()=1] publication-title: J. Nanobiotechnol. doi: 10.1186/s12951-015-0076-6 contributor: fullname: Weng – volume: 7 start-page: 19358 year: 2015 ident: C7NR04307D-(cit3)/*[position()=1] publication-title: Nanoscale doi: 10.1039/C5NR06144J contributor: fullname: Huang – volume: 19 start-page: 140 year: 2016 ident: C7NR04307D-(cit31)/*[position()=1] publication-title: Mater. Today doi: 10.1016/j.mattod.2015.08.019 contributor: fullname: Presolski – volume: 7 start-page: 15953 year: 2015 ident: C7NR04307D-(cit55)/*[position()=1] publication-title: Nanoscale doi: 10.1039/C5NR02224J contributor: fullname: Jia – volume: 25 start-page: 5086 year: 2015 ident: C7NR04307D-(cit18)/*[position()=1] publication-title: Adv. Funct. Mater. doi: 10.1002/adfm.201500891 contributor: fullname: Kalantar-zadeh – volume: 939 start-page: 101 year: 2016 ident: C7NR04307D-(cit29)/*[position()=1] publication-title: Anal. Chim. Acta doi: 10.1016/j.aca.2016.08.010 contributor: fullname: Kukkar – volume: 83 start-page: 339 year: 2016 ident: C7NR04307D-(cit48)/*[position()=1] publication-title: Biosens. Bioelectron. doi: 10.1016/j.bios.2016.04.058 contributor: fullname: Mehta – volume: 4 start-page: 2695 year: 2010 ident: C7NR04307D-(cit46)/*[position()=1] publication-title: ACS Nano doi: 10.1021/nn1003937 contributor: fullname: Lee – volume: 22 start-page: 1385 year: 2012 ident: C7NR04307D-(cit47)/*[position()=1] publication-title: Adv. Funct. Mater. doi: 10.1002/adfm.201102111 contributor: fullname: Li |
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nanostructure-based disposable electrochemical immunosensor to detect β-hydroxybutyrate (βHBA), a novel... We report the step-by-step fabrication of a 2D MoS 2 nanostructure-based disposable electrochemical immunosensor to detect β-hydroxybutyrate (βHBA), a novel... |
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| SubjectTerms | 3-Hydroxybutyric Acid - analysis Animals Biomarkers - analysis Biosensing Techniques Electrochemical Techniques Electrodes Gold Humans Immunoassay Ketosis - diagnosis Limit of Detection Nanostructures |
| Title | Liquid exfoliation of 2D MoS 2 nanosheets and their utilization as a label-free electrochemical immunoassay for subclinical ketosis |
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