Liquid exfoliation of 2D MoS 2 nanosheets and their utilization as a label-free electrochemical immunoassay for subclinical ketosis

We report the step-by-step fabrication of a 2D MoS nanostructure-based disposable electrochemical immunosensor to detect β-hydroxybutyrate (βHBA), a novel subclinical ketosis biomarker. The MoS nanosheets were exfoliated in the liquid phase by ultrasonication, and then followed by deposition on gold...

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Published inNanoscale Vol. 9; no. 30; pp. 10886 - 10896
Main Authors Tuteja, Satish K, Duffield, Todd, Neethirajan, Suresh
Format Journal Article
LanguageEnglish
Published England 03.08.2017
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Abstract We report the step-by-step fabrication of a 2D MoS nanostructure-based disposable electrochemical immunosensor to detect β-hydroxybutyrate (βHBA), a novel subclinical ketosis biomarker. The MoS nanosheets were exfoliated in the liquid phase by ultrasonication, and then followed by deposition on gold colloid modified screen-printed electrodes (Au-SPE). The MoS -modified electrodes were thoroughly characterized by physical, electrochemical as well as spectroscopic techniques, and the obtained results indicate the successful and irreversible electrodeposition of MoS nanosheets. These MoS -modified disposable electrodes were subsequently bioconjugated with anti-βHBA antibodies and then employed for the label-free immunosensing of the βHBA biomarker to detect subclinical ketosis. A simple electrochemical Differential Pulse Voltammetry (DPV) technique based immunodetection was realized for the sensing of varying concentrations of βHBA antigen. The bioassay demonstrated remarkable sensitivity and specificity having a dynamic linear response range of 0.7 mM to 10 mM for βHBA in standard antigen solutions, spiked serum, blood, milk and clinical samples, with linear results being obtained with R ∼ 0.9923. The sensor electrodes were also studied with other relative interferents to investigate cross reactivity and non-specificity. These electrodes showed a linear, specific, reproducible and stable response towards the βHBA antigen over a wide range of concentrations.
AbstractList We report the step-by-step fabrication of a 2D MoS 2 nanostructure-based disposable electrochemical immunosensor to detect β-hydroxybutyrate (βHBA), a novel subclinical ketosis biomarker. The MoS 2 nanosheets were exfoliated in the liquid phase by ultrasonication, and then followed by deposition on gold colloid modified screen-printed electrodes (Au-SPE). The MoS 2 -modified electrodes were thoroughly characterized by physical, electrochemical as well as spectroscopic techniques, and the obtained results indicate the successful and irreversible electrodeposition of MoS 2 nanosheets. These MoS 2 -modified disposable electrodes were subsequently bioconjugated with anti-βHBA antibodies and then employed for the label-free immunosensing of the βHBA biomarker to detect subclinical ketosis. A simple electrochemical Differential Pulse Voltammetry (DPV) technique based immunodetection was realized for the sensing of varying concentrations of βHBA antigen. The bioassay demonstrated remarkable sensitivity and specificity having a dynamic linear response range of 0.7 mM to 10 mM for βHBA in standard antigen solutions, spiked serum, blood, milk and clinical samples, with linear results being obtained with R 2 ∼ 0.9923. The sensor electrodes were also studied with other relative interferents to investigate cross reactivity and non-specificity. These electrodes showed a linear, specific, reproducible and stable response towards the βHBA antigen over a wide range of concentrations.
We report the step-by-step fabrication of a 2D MoS nanostructure-based disposable electrochemical immunosensor to detect β-hydroxybutyrate (βHBA), a novel subclinical ketosis biomarker. The MoS nanosheets were exfoliated in the liquid phase by ultrasonication, and then followed by deposition on gold colloid modified screen-printed electrodes (Au-SPE). The MoS -modified electrodes were thoroughly characterized by physical, electrochemical as well as spectroscopic techniques, and the obtained results indicate the successful and irreversible electrodeposition of MoS nanosheets. These MoS -modified disposable electrodes were subsequently bioconjugated with anti-βHBA antibodies and then employed for the label-free immunosensing of the βHBA biomarker to detect subclinical ketosis. A simple electrochemical Differential Pulse Voltammetry (DPV) technique based immunodetection was realized for the sensing of varying concentrations of βHBA antigen. The bioassay demonstrated remarkable sensitivity and specificity having a dynamic linear response range of 0.7 mM to 10 mM for βHBA in standard antigen solutions, spiked serum, blood, milk and clinical samples, with linear results being obtained with R ∼ 0.9923. The sensor electrodes were also studied with other relative interferents to investigate cross reactivity and non-specificity. These electrodes showed a linear, specific, reproducible and stable response towards the βHBA antigen over a wide range of concentrations.
Author Tuteja, Satish K
Neethirajan, Suresh
Duffield, Todd
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Snippet We report the step-by-step fabrication of a 2D MoS nanostructure-based disposable electrochemical immunosensor to detect β-hydroxybutyrate (βHBA), a novel...
We report the step-by-step fabrication of a 2D MoS 2 nanostructure-based disposable electrochemical immunosensor to detect β-hydroxybutyrate (βHBA), a novel...
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StartPage 10886
SubjectTerms 3-Hydroxybutyric Acid - analysis
Animals
Biomarkers - analysis
Biosensing Techniques
Electrochemical Techniques
Electrodes
Gold
Humans
Immunoassay
Ketosis - diagnosis
Limit of Detection
Nanostructures
Title Liquid exfoliation of 2D MoS 2 nanosheets and their utilization as a label-free electrochemical immunoassay for subclinical ketosis
URI https://www.ncbi.nlm.nih.gov/pubmed/28731116
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