Liquid exfoliation of 2D MoS 2 nanosheets and their utilization as a label-free electrochemical immunoassay for subclinical ketosis
We report the step-by-step fabrication of a 2D MoS nanostructure-based disposable electrochemical immunosensor to detect β-hydroxybutyrate (βHBA), a novel subclinical ketosis biomarker. The MoS nanosheets were exfoliated in the liquid phase by ultrasonication, and then followed by deposition on gold...
Saved in:
Published in | Nanoscale Vol. 9; no. 30; pp. 10886 - 10896 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
England
03.08.2017
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | We report the step-by-step fabrication of a 2D MoS
nanostructure-based disposable electrochemical immunosensor to detect β-hydroxybutyrate (βHBA), a novel subclinical ketosis biomarker. The MoS
nanosheets were exfoliated in the liquid phase by ultrasonication, and then followed by deposition on gold colloid modified screen-printed electrodes (Au-SPE). The MoS
-modified electrodes were thoroughly characterized by physical, electrochemical as well as spectroscopic techniques, and the obtained results indicate the successful and irreversible electrodeposition of MoS
nanosheets. These MoS
-modified disposable electrodes were subsequently bioconjugated with anti-βHBA antibodies and then employed for the label-free immunosensing of the βHBA biomarker to detect subclinical ketosis. A simple electrochemical Differential Pulse Voltammetry (DPV) technique based immunodetection was realized for the sensing of varying concentrations of βHBA antigen. The bioassay demonstrated remarkable sensitivity and specificity having a dynamic linear response range of 0.7 mM to 10 mM for βHBA in standard antigen solutions, spiked serum, blood, milk and clinical samples, with linear results being obtained with R
∼ 0.9923. The sensor electrodes were also studied with other relative interferents to investigate cross reactivity and non-specificity. These electrodes showed a linear, specific, reproducible and stable response towards the βHBA antigen over a wide range of concentrations. |
---|---|
ISSN: | 2040-3364 2040-3372 |
DOI: | 10.1039/C7NR04307D |