Polyoxometalates as artificial nucleases: hydrolytic cleavage of DNA promoted by a highly negatively charged Zr IV -substituted Keggin polyanion

A highly negatively charged binuclear Zr -substituted Keggin polyoxometalate [{α-PW O Zr(μ-OH)(H O)} ] (ZrK 2 : 2) has been shown to promote the hydrolytic cleavage of phosphoester bonds in the supercoiled plasmid pUC19 DNA under physiological pH and temperature, giving relaxed and linear forms of p...

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Published inChemical communications (Cambridge, England) Vol. 53; no. 3; pp. 617 - 620
Main Authors Luong, T K N, Govaerts, I, Robben, J, Shestakova, P, Parac-Vogt, T N
Format Journal Article
LanguageEnglish
Published England 03.01.2017
Subjects
DNA Cleavage
DNA, Superhelical - chemistry
Hydrolysis
Polymers - chemistry
Tungsten Compounds - chemistry
Zirconium - chemistry
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Summary:A highly negatively charged binuclear Zr -substituted Keggin polyoxometalate [{α-PW O Zr(μ-OH)(H O)} ] (ZrK 2 : 2) has been shown to promote the hydrolytic cleavage of phosphoester bonds in the supercoiled plasmid pUC19 DNA under physiological pH and temperature, giving relaxed and linear forms of pUC19 as hydrolysis products. The interaction between ZrK 2 : 2 and DNA was experimentally proven by circular dichroism (CD) spectroscopy and P diffusion ordered NMR spectroscopy.
ISSN:1359-7345
1364-548X
DOI:10.1039/C6CC08555E