Abstract 5163: MMP3 increases expression of CXCL7

• Published in: Cancer research (Chicago, Ill.) Vol. 73; no. 8_Supplement; p. 5163
• Main Authors: Ju, Donghong, Kosir, Mary A.
• Format: Journal Article
• Language: English
• Published: 15.04.2013
• ISSN: 0008-5472; 1538-7445
• DOI: 10.1158/1538-7445.AM2013-5163

Abstract MMP3 leads to epithelial-mesenchymal transformation (EMT) and malignant transformation of cultured cells. Previously, increased expression of the CXC chemokine CXCL7 was associated with EMT. The hypothesis tested is that MMP3 increases the expression of CXCL7 Methods: Human breast cells (MCF10AT (proliferative), MCFDCIS.com (in situ), MCFCA1a (malignant)) cultured in serum free medium were exposed to MMP-3 for 4 days, and washed 3 times with PBS, then cultured in MMP-3 free condition medium for three more days. Cells were collected every day for RNA extraction, and analyzed for relative CXCL7 gene expression using RT real time PCR. Cells sampled from day 0 were used as control to normalize the results. GADPH was control. Cells culture supernatant also collected every day for CXCL7 protein level expression. Protein concentrations were measured by enzyme-linked immunosorbent assay (ELISA) using human CXCL7/NAP-2 Duo set ELISA Development System. Measurements were done in triplicate; results are representative of three experiments. Results: When exposed to MMP-3, the CXCL7 RNA level increased MCF-10AT cells RNA level increased 1.5 fold, and there was no change in CXCL7 protein level in MCF-10AT cells. For MCFDCIS.com cells, the CXCL7 RNA level increased 2.5 fold, and CXCL7 protein level increased 1.6 fold. For MCFCA1a cells, the CXCL7 RNA level increased 2.6 fold, CXCL7 protein level increased 1.5 fold. The peak expression was 3-4 days. Removal of MMP-3 caused CXCL7 expression to return back to baseline in MCF10AT, DCIS.com and malignant Ca.cl1 cells. Conclusion: The expression of chemokine CXCL7 in breast cell (MCF10AT, DCIS.com, MCFCA1a) was up regulated at both on RNA and protein level (except for MCF10AT) when exposed to MMP-3, and removal of MMP-3 caused CXCL7 expression to return to baseline. The stromal influence by MMP3 on breast cells and breast cancer cells may direct further progression toward the malignant phenotype and provides another therapeutic pathway target. Citation Format: Donghong Ju, Mary A. Kosir. MMP3 increases expression of CXCL7. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5163. doi:10.1158/1538-7445.AM2013-5163