Lipid droplets and gallbladder targeted fluorescence probe for ratiometric NO imaging in gallstones disease models

• Published in: Chinese chemical letters p. 110191
• Main Authors: Zheng, Zhoupeng, Gong, Shengyi, Li, Qianhua, Zhang, Shiya, Feng, Guoqiang
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.06.2024
• Subjects: Gallbladder; Gallstone disease; Lipid droplets; Nitric oxide; Ratiometric fluorescent probe; Gallbladder; Ratiometric fluorescent probe; Gallstone disease; Lipid droplets; Nitric oxide
• ISSN: 1001-8417; 1878-5964
• DOI: 10.1016/j.cclet.2024.110191

Gallstones are a common disease worldwide, often leading to obstruction and inflammatory complications, which seriously affect the quality of life of patients. Research has shown that gallstone disease is associated with ferroptosis, lipid droplets (LDs), and abnormal levels of nitric oxide (NO). Fluorescent probes provide a sensitive and convenient method for detecting important substances in life systems and diseases. However, so far, no fluorescent probes for NO and LDs in gallstone disease have been reported. In this work, an effective ratiometric fluorescent probe LR-NH was designed for the detection of NO in LDs. With an anthracimide fluorophore and a secondary amine as a response site for NO, LR-NH exhibits high selectivity, sensitivity, and attractive ratiometric capability in detecting NO. Importantly, it can target LDs and shows excellent imaging ability for NO in cells and ferroptosis. Moreover, LR-NH can target the gallbladder and image NO in gallstone disease models, providing a unique and unprecedented tool for studying NO in LDs and gallbladder. A lipid droplets and gallbladder targeted fluorescent probe was reported for ratiometric fluorescence imaging of NO in cells and gallbladder, revealing an increased level of NO in gallstone diseases. [Display omitted]