共反应试剂增强电致化学发光信号生物传感器

在电致化学发光（ECL）生物传感器的构建中，利用共反应试剂促进发光基团的发光效率是一种常见、方便且非常有效的方法．然而．如何更好地利用共反应试剂使其更加有效地与发光基团作用是提高该类生物传感器灵敏度的重要因素．本文结合作者课题组部分工作综述了三种共反应试剂放大ECL信号的构建：共反应试剂内置于检测底液；共反应试剂共存于电极表面；酶促生成共反应试剂，并提出了今后ECL信号放大构建的展望．...

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Bibliographic Details
Published in电化学 Vol. 21; no. 1; pp. 13 - 21
Main Author 王海军 肖丽娟 何颖 蒋欣亚 袁亚利 卓颖 柴雅琴 袁若
Format Journal Article
LanguageChinese
Published 28.02.2015
Subjects
信号放大
共反应试剂
生物传感器
电致化学发光
Online AccessGet full text
ISSN1006-3471
2993-074X
DOI10.13208/j.electrochem.140439

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Summary:在电致化学发光（ECL）生物传感器的构建中，利用共反应试剂促进发光基团的发光效率是一种常见、方便且非常有效的方法．然而．如何更好地利用共反应试剂使其更加有效地与发光基团作用是提高该类生物传感器灵敏度的重要因素．本文结合作者课题组部分工作综述了三种共反应试剂放大ECL信号的构建：共反应试剂内置于检测底液；共反应试剂共存于电极表面；酶促生成共反应试剂，并提出了今后ECL信号放大构建的展望．
Bibliography:WANG Hai-jun, XIAO Li-juan, HE Ying, JIANG Xin-ya, YUAN Ya-li, ZHUO Ying, CHAI Ya-qin, YUAN Ruo （College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China）
35-1172/06
Using co-reactants to enhance the luminous efficiency of luminophores is a common, convenient and effective method in the construction of the electrochemiluminescence （ECL） biosensors. However, the different methods of introducing co-reactants in the construction of the ECL biosensors will bring different amplification effects. In this review, several signal amplification methods through co-reactants are summarized： 1） Adding the co-reactants directly into the detection solution; 2） Immobilizing the co-reactants onto the electrode surface; 3） Using enzymatic reaction to generate co-reactants in situ around the electrode surface. And then, the outlook of the ECL signal amplification strategy is proposed on those bases.
electrochemiluminescence; co-reactants; amplification; biosensors
ISSN:1006-3471
2993-074X
DOI:10.13208/j.electrochem.140439