Expression of extracellular human prolactin in insect cells

Human prolactin was expressed in the insect culture cells as a soluble extracellular protein using recombinant baculoviruses. The yield varied between 20-30 mg/l medium. This recombinant prolactin was biologically active in the Nb2 rat lymphoma cells proliferation assay.

Saved in:
Bibliographic Details
Published inBiopolimery i kletka Vol. 20; no. 4; pp. 325 - 330
Main Authors Strokovskaya, L. I., Kikhno, I. M., Meleshko, R. A., Solomko, A. P.
Format Journal Article
LanguageEnglish
Published Kiev Natsional'na Akademiya Nauk Ukrainy - National Academy of Sciences of Ukraine 20.07.2004
Subjects
Biological activity
Cell culture
Cell proliferation
Insect cells
Lymphoma
Prolactin
Online AccessGet full text

Cover

More Information
Summary:Human prolactin was expressed in the insect culture cells as a soluble extracellular protein using recombinant baculoviruses. The yield varied between 20-30 mg/l medium. This recombinant prolactin was biologically active in the Nb2 rat lymphoma cells proliferation assay.
ISSN:0233-7657
1993-6842
DOI:10.7124/bc.0006B7