Wide field-of-view, twin-region two-photon imaging across extended cortical networks

We demonstrate a two-photon imaging system with corrected optics including a custom objective that provides cellular resolution across a 3.5 mm field of view (9.6 mm^2). Temporally multiplexed excitation pathways can be independently repositioned in XY and Z to simultaneously image regions within th...

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Published inbioRxiv
Main Authors Stirman, Jeffrey N, Smith, Ikuko T, Kudenov, Michael W, Smith, Spencer L
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 08.07.2015
Subjects
Cortex
Optics
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Abstract We demonstrate a two-photon imaging system with corrected optics including a custom objective that provides cellular resolution across a 3.5 mm field of view (9.6 mm^2). Temporally multiplexed excitation pathways can be independently repositioned in XY and Z to simultaneously image regions within the expanded field of view. We used this new imaging system to measure activity correlations between neurons in different cortical areas in awake mice.
AbstractList We demonstrate a two-photon imaging system with corrected optics including a custom objective that provides cellular resolution across a 3.5 mm field of view (9.6 mm^2). Temporally multiplexed excitation pathways can be independently repositioned in XY and Z to simultaneously image regions within the expanded field of view. We used this new imaging system to measure activity correlations between neurons in different cortical areas in awake mice.
Author Smith, Spencer L
Smith, Ikuko T
Stirman, Jeffrey N
Kudenov, Michael W
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CitedBy_id crossref_primary_10_7554_eLife_14472
crossref_primary_10_7554_eLife_14679
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Copyright 2015. Notwithstanding the ProQuest Terms and conditions, you may use this content in accordance with the associated terms available at https://www.biorxiv.org/content/early/2015/07/08/011320
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