Wide field-of-view, twin-region two-photon imaging across extended cortical networks

We demonstrate a two-photon imaging system with corrected optics including a custom objective that provides cellular resolution across a 3.5 mm field of view (9.6 mm^2). Temporally multiplexed excitation pathways can be independently repositioned in XY and Z to simultaneously image regions within th...

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Bibliographic Details
Published inbioRxiv
Main Authors Stirman, Jeffrey N, Smith, Ikuko T, Kudenov, Michael W, Smith, Spencer L
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 08.07.2015
Subjects
Cortex
Optics
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Summary:We demonstrate a two-photon imaging system with corrected optics including a custom objective that provides cellular resolution across a 3.5 mm field of view (9.6 mm^2). Temporally multiplexed excitation pathways can be independently repositioned in XY and Z to simultaneously image regions within the expanded field of view. We used this new imaging system to measure activity correlations between neurons in different cortical areas in awake mice.
DOI:10.1101/011320