An experimental study of a modified Dahuang Zhechong pill on theangiogenesis of RF/6A cells in vitro

• Published in: 中医杂志：英文版 Vol. 32; no. 1; pp. 75 - 81
• Main Author: 罗旭昇 吴星伟 顾青
• Format: Journal Article
• Language: English
• Published: 2012
• Subjects: MMP-2; mRNA表达; VEGF; 内皮细胞; 反转录聚合酶链反应; 大黄; 血管内皮生长因子; 金属蛋白酶-2
• ISSN: 0255-2922
• DOI: 10.1016/s0254-6272(12)60036-3

OBJECTIVE：To investigate the effects of a modified Dahuang Zhechong Pill（MDZP） on the angiogene sis of rhesus choroid-retina endothelial（RF/6A cells and its preliminary mechanism.METHODS：A 3-（4,5-dimethylthiazol-2-yl）-5-（3-car boxymethoxyphenyl）-2-（4-sulfophenyl）-2H-tetrazol ium（MTS） method was used to assess the effect o a MDZP on RF/6A cell proliferation induced by vas cular endothelial growth factor（VEGF）.Transwell in serts were used to assess the effect of the MDZP on RF/6A cell migration.Matrigel was used to asses the effect of the MDZP on the tube formation of RF 6A cells.Western blotting and quantitative re al-time reverse transcription polymerase chain reac tion（RT-PCR） were used to detect the protein and mRNA expression,respectively,of VEGF and matri metalloproteinase-2（MMP-2） in RF/6A cells treatedwith the MDZP.RESULTS：RF/6A cell proliferation induced by VEGF was inhibited by 0.2 mg/mL MDZP.At 0,12.5,25 and 50 mg/mL MDZP,the number of cells that migrated through Transwell membranes was 73.33± 4.51,61.33±4.04,28.67±6.66 and 17.67±4.16,respectively,and the number of tubes formed in Matrigel was 20.33±0.58,13.33±1.53,11.00±1.00 and 1.33±0.58,respectively.At 100 and 200 mg/mL MDZP,the protein and mRNA expression of VEGF and MMP-2 were inhibited in RF/6A cells.At 400 mg/mL MDZP,the expression of VEGF mRNA and MMP-2 protein were inhibited in RF/6A cells.CONCLUSIONS：MDZP inhibits the angiogenesis of RF/6A cells via the suppression of proliferation,migration and tube formation of RF/6A cells.Inhibition of the protein and mRNA expression of VEGF and MMP-2 in RF/6A cells may be an important mechanism.