Comprehensive chemical profiling with UHPLC-MS, in-vitro, in-silico, and in-vivo antidiabetic potential of Typha domingensis Pers; A novel source of bioactive compounds

• Published in: South African journal of botany Vol. 171; pp. 185 - 198
• Main Authors: Dilshad, Rizwana, Khan, Kashif-ur-Rehman, Dilshad, Rihana, Ahmad, Saeed, Rao, Huma, Khurshid, Umair, Ahmad, Sultan, Ahmad, Maqsood, Abid, Hafiz Muhammad Usman, Zaman, Muhammad Kashif, Nisar, Rabia, Khaliq, Saharish, Ghalloo, Bilal Ahmad
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.08.2024
• Subjects: Anti-diabetes; Antioxidant; Enzyme inhibition; In-silico studies; LCMS; n-butanol fraction; Typha domingensis Pers; HPLC-PDA; TTC; DMSO; UHPLC-MS; In-silico studies; Typha domingensis Pers; Antioxidant; EDTA; ADMET; Anti-diabetes; n-butanol fraction; TDBF; Enzyme inhibition; Log Po/w; Mass Q-TOF; mg TAEq.g−1 DE; LCMS
• ISSN: 0254-6299; 1727-9321
• DOI: 10.1016/j.sajb.2024.06.007

•A literature survey revealed that information about antidiabetic activities of Typha domingensis is not available.•For the first time, in-vitro, in-silico, and in-vivo antidiabetic activities were performed.•Chemical profiling was done with UHPLC-MS and HPLC-PDA.•The study provides scientific evidence that n-butanol fraction of T. domingensis has strong antidiabetic effects and after evaluation it can be used for treating diabetes. Typha is one of the valuable plant genera in folklore use in diabetes, and it can be a good source of bioactive chemicals. The current project was planned to investigate the pharmaceutical potential of n-butanol fraction of Typha domingensis Pers. (TDBF), a less explored medicinal plant. The phytochemical composition of the TDBF was assessed through a comprehensive analysis, encompassing the quantification of total bioactive contents (including total tannins and HPLC-PDA quantification) and a thorough exploration of secondary metabolites through scanning with UHPLC-MS. The pharmacological assessment included the determination of antioxidant activity (Total Antioxidant Capacity and Metal Chelating Assay), hemolytic potential, in-vitro antidiabetic effects (α-glucosidase and α-amylase inhibition, and antiglycation activity), as well as in-vivo antidiabetic activities. Molecular docking and ADMET studies were also performed for UHPLC-MS identified compounds. The TDBF showed 134.76 ± 8.23 mg TAE/g total tannin contents. With HPLC-PDA, p-Hydroxy benzoic acid and p-Coumaric acid were quantified. During UHPLC-MS scanning, a total of five bioactive metabolites were identified in the negative mode, while 54 were detected in the positive mode. The in-vivo assessment of antidiabetic activity revealed a notable reduction in blood glucose levels in diabetic rats following treatment with the extract, demonstrating its efficacy compared to the control groups. Furthermore, the extract exhibited a positive impact on renal and liver function, evidenced by lowered levels of serum urea, ALT, ALP, creatinine, and a decrease in serum cholesterol levels. The results of this study showed that Typha domingensis Pers. possesses a variety of bioactive compounds and demonstrates notable potential in regulating diabetes. The study recommends further investigation into isolating these bioactive phytochemicals for their potential therapeutic applications across various diseases.