EFFECT OF INTRAORAL OR EXTRAORAL PHOTOBIOMODULATION ON ORAL MUCOSITIS INDUCED BY CHEMOTHERAPY IN RATS

• Published in: Oral surgery, oral medicine, oral pathology and oral radiology Vol. 130; no. 3; p. e278
• Main Authors: THIEME, STéFANIE, RIBEIRO, JULIA TURRA, ZIEGER, RENATA DE ALMEIDA, SEVERO, MARA LUANA BATISTA, MARTINS, MARCO ANTONIO TREVIZANI, MATTÉ, CRISTIANE, MARTINS, MANOELA DOMINGUES
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.09.2020
• ISSN: 2212-4403; 2212-4411
• DOI: 10.1016/j.oooo.2020.04.765

Purpose: To compare the effect of intraoral (IO) and extraoral (EO) diode laser irradiation on oral mucositis (OM) induced by 5- fluorouracil (5-FU) in rats. Study Design: Seventy-eight male Wistar rats were divided into the following groups: negative control (NC), positive control (PC), IO 6 J/cm2, EO with 6 J/cm2, and EO with 12 J/cm2. OM was induced with an intraperitoneal injection of 5-FU and scarification of the buccal mucosa. Animals received photobiomodulation (PBM) daily. Clinical, histologic, and redox state evaluations were performed at days 8, 10, and 14. Results: Clinically, on day 8, EO 6 J/cm2 showed a lower score compared to PC (P< .05). On days 10 and 14, all PBM groups exhibited improvement of OM compared to PC (P< .01). Histologically, on day 8, all PBM groups exhibited an accelerated healing process compared to PC (P< .01) and reduction of reactive species (P< .001). Also, all PBM groups demonstrated higher levels of antioxidant GPx compared to PC (P< .001). Conclusions: An EO diode laser protocol promoted positive effects in the clinical, histopathological, and redox state in OM induced by 5-FU in rats. Among the EO protocols, EO 6 J/cm2 showed the most encouraging results.