In vitro development of macrophages from PHA-stimulated blood cultures in Hodgkin's disease and in chronic lymphocytic leukaemia (author's transl)

Blood was cultured from 17 normal subjects, 28 cases of untreated chronic lymphocytic leukaemia (CLL) and 41 cases of Hodgkin's disease. Macrophages were not observed in PHA-stimulated cultures of normal subjects, of CLL or of 18 cases of Hodgkin's disease. The latter had an almost normal...

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Bibliographic Details
Published inTumori Vol. 62; no. 1; p. 19
Main Authors Navone, R, Tosto, M, Poggio, E
Format Journal Article
LanguageEnglish
Italian
Published United States 01.01.1976
Subjects
Antibody Formation
Hodgkin Disease - immunology
Humans
Lectins
Leukemia, Lymphoid - immunology
Lymphocyte Activation
Macrophages - immunology
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Summary:Blood was cultured from 17 normal subjects, 28 cases of untreated chronic lymphocytic leukaemia (CLL) and 41 cases of Hodgkin's disease. Macrophages were not observed in PHA-stimulated cultures of normal subjects, of CLL or of 18 cases of Hodgkin's disease. The latter had an almost normal rate of lymphocyte blast transformation (70,2 +/- 7,2%).on the other hand, macrophages were numerous in PHA-stimulated cultures of 23 cases of Hodgkin's disease with a low blast transformation (28.4 +/- 13,7%). In Hodgkin's disease the development of macrophages in PHA cultures may be related to the lower blast transformation and cytotoxic activity of lymphocytes. In CLL the dilution of lymphocytes and monocytes by leukaemic B cells may account both for PHA unresponsiveness and low yield of macrophages in culture.
ISSN:0300-8916
DOI:10.1177/030089167606200103