WOUND HEALING ACTIVITY OF A GELATIN-BASED HYDROGEL CONTAINING 2% ETHANOLIC EXTRACT OF HIMATANTHUS BRACTEATUS

• Published in: Oral surgery, oral medicine, oral pathology and oral radiology Vol. 134; no. 3; p. e225
• Main Authors: Silva, João Vitor Rocha, De Almeida, Bernadeth Moda, Santos, Izabella Dayana Dorta Dos, Cunha, John Lennon Silva, De Almeida, Oslei Paes, Cardoso, Juliana Cordeiro, De Albuquerque, Ricardo Luiz Cavalcanti
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.09.2022
• ISSN: 2212-4403; 2212-4411
• DOI: 10.1016/j.oooo.2022.01.713

To analyze the effect of topical application of a photopolymerizable hydrogel based on methacrylated gelatin containing 2% ethanolic extract of Himatanthus bracteatus (GelMA-HB) on wound healing in rodents. Standard-sized (0.8 cm diameter) round-shaped wounds were made in the back of Wistar rats treated with photopolymerizable hydrogel based on methacrylated gelatin (GelMA), GelMA-HB, and saline (CTR). The animals were euthanized after 3, 7, and 14 days. Wound retraction index (WRi), wound healing histologic grading (WHHG), and immunohistochemical expression of α-SMA and CD105 were assessed for myofibroblastic differentiation (MFd) and microvascular density (MVd) analysis, respectively. The WRi and WHHG in GelMA-HB were significantly greater than in GelMA (P < .05) and CTR (P < .01) over the full time course of the experiment. Both the MFd and MVd were greater in GelMA-HB than in CTR on day 3 (P < .05) and day 7 (P < .001). The GelMA-based formulation containing ethanolic extract of H. bracteatus improved wound healing in a rodent model, and such biological effects might be related to the modulation of myofibroblastic and microvascular differentiation over the course of wound healing.