EFFECT OF TOPICAL PLATELET-DERIVED EXTRACELLULAR VESICLE THERAPY IN ATOPIC DERMATITIS

• Published in: Cytotherapy (Oxford, England) Vol. 26; no. 6; p. S72
• Main Authors: Jeldres, M.E. Carrasco, Gudapati, H., Inman, C., Weston, A., Yu, G., Behfar, A., Wyles, S.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.06.2024
• Subjects: Healing; Regeneration; Skin; Regeneration; Healing; Skin
• ISSN: 1465-3249; 1477-2566
• DOI: 10.1016/j.jcyt.2024.03.130

Atopic dermatitis (AD) is the most common chronic inflammatory skin disease. AD is seen in approximately 10% to 30% of children and 2% to 10% of adults in developed countries. AD etiology including genetic and environmental factors which lead to abnormalities in the epidermis and the immune system. In AD, the major components of treatment include trigger avoidance, daily skin care, anti-inflammatory therapy, and other complementary modalities. Extracellular vesicle (EVs) are membrane-enclosed structures released by cells that mediate intercellular communication. EVs have been assigned multiple functions that have established their potential as therapeutic mediators for a variety of diseases and conditions. In this study, we evaluated the effect of topical platelet derived EVs in AD skin repair and regeneration. Male and female hairless mice 4 months old were sensitized with 5% Oxazolone (OXA) on the dorsal skin and followed by challenges 1 week later with 0.1% OXA every other day for 2 more weeks. Then, mice were treated with a topical application of 20% topical platelet EVs cream base or 0.05% clobetasol (Clob) as a positive control treatment twice a day for 10 days consecutives under OXA induction (Fig 1A). A single topical treatment with OXA after the first sensitization follows for repeated challenges of OXA is sufficient to induce AD mouse model (Fig 1B). H&E staining of skin biopsy samples reveals dermal-epidermal inflammatory infiltrate and epidermal hyperplasia in AD mouse models (Fig 1C). Reduction in epidermal hyperplasia and inflammation was observed in mice following topical platelet EV treatment (Fig 1D). This study shows that topical platelet EV treatment can reduce histological alternations associated to AD to reduce inflammation and improve skin repair.