Spirulina (Arthrospira platensis) Extract Promotes Motility, Microscopic, and Antioxidative Parameters of Ram Semen during Refrigerated Storage

This study investigated the effect of spirulina ethanolic extract (SEE) on the quality of ram semen during low-temperature liquid storage and the relationship between sperm features. Ejaculates were collected from five Djallonké rams, pooled, extended with Tris-egg yolk (TEY) enriched with 0 (contro...

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Published inBlack Sea Journal of Agriculture Vol. 5; no. 4; pp. 481 - 490
Main Authors KAMENİ LEUGOUÉ, Serge, ARİUS BAULLAND, Dongmo Nguedıa, THOMAS TUMASANG, Tebug, FRANCİS DESİRE, Bomba Tatsınkou, MEUTCHİEYE, Félix, FERDİNAND, Ngoula
Format Journal Article
LanguageEnglish
Published 01.10.2022
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Summary:This study investigated the effect of spirulina ethanolic extract (SEE) on the quality of ram semen during low-temperature liquid storage and the relationship between sperm features. Ejaculates were collected from five Djallonké rams, pooled, extended with Tris-egg yolk (TEY) enriched with 0 (control), 20 (SEE20), 40 (SEE40), and 80 µg/mL (SEE80) of SEE to reach the concentration of 200×106 spz/mL, and stored at 4 °C for 72 h. Extended semen samples were assessed for total motility, progressive motility, sperm motion characteristics, viability, membrane integrity, and morphology at 6, 24, 48 and 72 h of storage. Moreover, malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase, (SOD) and catalase (CAT) levels were measured at 72 h of storage. The enrichment of TEY with SEE at 40 and 80 µg/mL, improved sperm total motility at 48 and 72 h of storage (P
ISSN:2618-6578
2618-6578
DOI:10.47115/bsagriculture.1162523