Isolation of IgG from chicken serum using a combination of coconut caprylic triglyceride and ammonium sulfate precipitation

This study aimed to isolate IgG from chicken serum using a combination of coconut caprylic triglyceride and ammonium sulfate precipitation techniques. This study used serum from chickens that had previously been vaccinated repeatedly using a commercial rabies vaccine. Initially, the serum was treate...

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Published inGSC biological and pharmaceutical sciences Vol. 27; no. 2; pp. 302 - 306
Main Authors Sulaiman Ngongu Depamede, Made Sriasih, Adji Santoso Sudradjat, Nurul Azizah
Format Journal Article
LanguageEnglish
Published 30.05.2024
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Abstract This study aimed to isolate IgG from chicken serum using a combination of coconut caprylic triglyceride and ammonium sulfate precipitation techniques. This study used serum from chickens that had previously been vaccinated repeatedly using a commercial rabies vaccine. Initially, the serum was treated with 2.5% coconut caprylic triglyceride, followed by serum IgG precipitation using 40% ammonium sulfate. The 10% SDS-PAGE analysis showed that a protein of about 190 kDa with a purity above 95% was successfully isolated. Whether the isolate protein is IgG and specific to rabies antigen still needs to be investigated further.
AbstractList This study aimed to isolate IgG from chicken serum using a combination of coconut caprylic triglyceride and ammonium sulfate precipitation techniques. This study used serum from chickens that had previously been vaccinated repeatedly using a commercial rabies vaccine. Initially, the serum was treated with 2.5% coconut caprylic triglyceride, followed by serum IgG precipitation using 40% ammonium sulfate. The 10% SDS-PAGE analysis showed that a protein of about 190 kDa with a purity above 95% was successfully isolated. Whether the isolate protein is IgG and specific to rabies antigen still needs to be investigated further.
Author Sulaiman Ngongu Depamede
Adji Santoso Sudradjat
Nurul Azizah
Made Sriasih
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