Isolation of IgG from chicken serum using a combination of coconut caprylic triglyceride and ammonium sulfate precipitation

This study aimed to isolate IgG from chicken serum using a combination of coconut caprylic triglyceride and ammonium sulfate precipitation techniques. This study used serum from chickens that had previously been vaccinated repeatedly using a commercial rabies vaccine. Initially, the serum was treate...

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Bibliographic Details
Published inGSC biological and pharmaceutical sciences Vol. 27; no. 2; pp. 302 - 306
Main Authors Sulaiman Ngongu Depamede, Made Sriasih, Adji Santoso Sudradjat, Nurul Azizah
Format Journal Article
LanguageEnglish
Published 30.05.2024
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Summary:This study aimed to isolate IgG from chicken serum using a combination of coconut caprylic triglyceride and ammonium sulfate precipitation techniques. This study used serum from chickens that had previously been vaccinated repeatedly using a commercial rabies vaccine. Initially, the serum was treated with 2.5% coconut caprylic triglyceride, followed by serum IgG precipitation using 40% ammonium sulfate. The 10% SDS-PAGE analysis showed that a protein of about 190 kDa with a purity above 95% was successfully isolated. Whether the isolate protein is IgG and specific to rabies antigen still needs to be investigated further.
ISSN:2581-3250
2581-3250
DOI:10.30574/gscbps.2024.27.2.0241