Estimation of the relative DNA content in species of the genus Spiraea, sections Chamaedryon and Glomerati by flow cytometry

The relative DNA content was studied in seven species of the genus Spiraea L., section Chamaedryon Ser., and in two species, section Glomerati Nakai, from 28 natural populations growing in Asian Russia. The cell nuclei were isolated from a leaf tissue. The relative intensity of fluorescence was meas...

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Published inUkrainian journal of ecology Vol. 9; no. 3; pp. 142 - 149
Main Authors Kostikova, V. A., Voronkova, M. S., Mitrenina, E. Yu, Kuznetsov, A. A., Ernst, A. S., Veklich, T. N., Shabanova(Kobozeva), E. V.
Format Journal Article
LanguageEnglish
Published Melitopol Bogdan Chmelnitskiy Melitopol State Pedagogical University 01.09.2019
Subjects
Cell cycle
Deoxyribonucleic acid
DNA
Ecology
Flow cytometry
Fluorescence
Genomes
Iodides
Laser radiation
Lasers
Leaves
Natural populations
Nuclei
Nucleus
Plant tissues
Propidium iodide
Radiation
Seeds
Species
Spiraea
Spiraea hypericifolia
Variance analysis
Germany
Russia
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Summary:The relative DNA content was studied in seven species of the genus Spiraea L., section Chamaedryon Ser., and in two species, section Glomerati Nakai, from 28 natural populations growing in Asian Russia. The cell nuclei were isolated from a leaf tissue. The relative intensity of fluorescence was measured using flow cytometry of propidium iodide-stained nuclei. The analysis was performed using a CyFlowSpace device (Germany, Sysmex Partec) with a laser radiation source of 532 nm. Fresh leaves of Solanum lycopersicum cv. ‘Stupice’ were used as an internal standard. Data on the relative DNA content are presented for the first time for S. flexuosa Fisch ex Cambess. (0.42–0.47 pg), S. ussuriensis Pojark. (0.49–0.52; 0.85 pg), S. alpina-Pall. (0.49–0.51 pg), S. media Schmidt. (0.45; 0.98–1.01 pg), S. trilobata L. (0.46 pg), S. hypericifolia L. (0.49–0.52 pg) and S. aquilegifolia Pall. (0.48–0.51 pg). Mesophytic species of the genus Spiraea growing in the forest zone (S. chamaedryfolia L. and S. media) exhibit a 2-fold higher relative DNA content (C-value) compared to xerophytic species (S. hypericifolia, S. crenata L., S. aquilegifolia and S. trilobata) and species confined only to mountain areas (S. alpinа, S. trilobata and S. aquilegifolia).
ISSN:2520-2138
2520-2138
DOI:10.15421/2019_71