Study on quality standard of Berberidis Cortex

• Published in: Zhongguo zhongyao zazhi Vol. 41; no. 4; p. 592
• Main Authors: Li, Yan, Lv, Xiu-Mei, Lin, Ya-Li, Mo, Jia-Qi, Fan, Gang, Lai, Xian-Rong, Zhang, Yi
• Format: Journal Article
• Language: Chinese; English
• Published: China 01.02.2016
• Subjects: Berberine Alkaloids - analysis; Berberis - chemistry; Chromatography, High Pressure Liquid - methods; Chromatography, Thin Layer - methods; Drugs, Chinese Herbal - analysis; Drugs, Chinese Herbal - standards; Quality Control; Berberidis Cortex; magnoflorine; palmatine; jatrorrhizine; quality standard; berberine
• DOI: 10.4268/cjcmm20160407
• ISSN: 1001-5302

In order to establish the quality standard of Berberidis Cortex and improve its quality control level, water, total ash, acid-insoluble ash and alcohol-soluble extract were determined according to procedures recorded in the Chinese Pharmacopoeia (2010 edition). The qualitative and quantitative analyses were performed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) methods. The results showed that TLC identification had a good resolution with clear spots. The water content was 8.39%-12.23%; total ash was 4.50%-9.96%; acid-insoluble ash was 0.10%-0.69%, and the alcohol-soluble extraction was 20.62%-37.13%. The average contents of magnoflorine, jatrorrhizine, palmatine, and berberine in Berberidis Cortex were 5.98%, 0.63%, 0.30%, 2.50%, respectively. It was concluded that the developed method was accurate and good in specificity, which can be used for quality control of Berberidis Cortex in the future.