Study of light signal receptor of Stephanopyxis palmeriana during sexual reproduction

• Published in: Chinese journal of oceanology and limnology Vol. 23; no. 3; pp. 330 - 334
• Main Author: 胡韧 林均民 林秋奇 韩博平
• Format: Journal Article
• Language: English
• Published: Research Center of Hydrobiology, Jinan University, Guangzhou 510632, China 2005; School of Life Science, Xiamen University, Xiamen 361005, China%School of Life Science, Xiamen University, Xiamen 361005, China%Research Center of Hydrobiology, Jinan University, Guangzhou 510632, China
• Subjects: Bacillariophyceae; Marine; Stephanopyxis palmeriana; 信号接受器; 光周期; 有性生殖; sexual reproduction; light signal receptor; diatom; photoperiod
• ISSN: 0254-4059; 1993-5005

We collected centric diatom Stephanopyxis palmeriana samples in coastal waters of Xiamen for characteristic red light/far red light (R/FR) phytochrome reactions to identify its photoreceptor in the course of sexual reproduction. The result showed that pre-illumination of 2-3h red light before darkness could induce sexualization of S.palmeriana, while the follow-up illumination of far red light could reverse the effect of red light, which is a featured reaction of phytochrome. The Southern Dot Blot was carried out to identify the type of phytochrome that induces the sexualization. The result also showed high homogeneity of DNA fragment of S. palmeriana with phyB, but phyA. This means the photoreceptor in the process of sexual reproduction of S. palmeriana is phytochrome B (phyB).