Development of bio-strip based test for ascertain proper milk pasteurization

Alkaline phosphatase (ALP) enzyme is naturally present in raw milk which is used as a prominent indicator to determine proper pasteurization in milk. However, determination of ALP activity in milk either time-consuming or required sophisticated instrument facilities that are uncommon in India's...

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Bibliographic Details
Published inJournal of Dairy, Veterinary & Animal Research Vol. 12; no. 1; pp. 61 - 64
Main Authors A Mangroliya, Parita, Hazra, Tanmay, G Sindhav, Rohit, K Ahuja, Kunal, Prasad, Subash
Format Journal Article
LanguageEnglish
Published 23.05.2023
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Summary:Alkaline phosphatase (ALP) enzyme is naturally present in raw milk which is used as a prominent indicator to determine proper pasteurization in milk. However, determination of ALP activity in milk either time-consuming or required sophisticated instrument facilities that are uncommon in India's rural dairy or collection centers. Present test is based on the ALP reaction with p-nitrophenyl phosphate to release p-nitrophenol and inorganic phosphate in the presence of water. When p-nitrophenol reacts with a certain chromogen, the strip's colour changes from purple to grey, which may be seen with the naked eye. This strip has a nine-month shelf life at room temperature.
ISSN:2377-4312
2377-4312
DOI:10.15406/jdvar.2023.12.00324