RFLP analysis of mitochondrial D-loop region in Indian sheep breeds

Genetic diversity was analyzed among six Indian sheep breeds using mitochondrial DNA(mt-DNA) D-loop region. A total of 216 blood samples from Deccani, Nellore, Malpura, Chokla, Nali and Sonadi sheep (36 of each) were assayed for restriction fragment length polymorphism (RFLP) with seven restriction...

Full description

Saved in:
Bibliographic Details
Published inIndian journal of small ruminants Vol. 21; no. 1; pp. 13 - 16
Main Authors Sahare, M.G, Sawaimul, A.D, Ali, S.Z, Sirothia, A.R, Kumar, S
Format Journal Article
LanguageEnglish
Published Indian Society for Sheep and Goat Production and Utilization 01.04.2015
Subjects
Genetic diversity
mt-DNA
Phylogenetic relationship
RFLP-PCR
Sheep breeds
Online AccessGet full text

Cover

More Information
Summary:Genetic diversity was analyzed among six Indian sheep breeds using mitochondrial DNA(mt-DNA) D-loop region. A total of 216 blood samples from Deccani, Nellore, Malpura, Chokla, Nali and Sonadi sheep (36 of each) were assayed for restriction fragment length polymorphism (RFLP) with seven restriction enzymes. The estimated genetic distance among the breeds ranged from 0.0658 to 0.1321. Deccani and Malpura sheep have the highest genetic distance between them whereas Chokla and Nali breed has the lowest. Phylogenetic analysis showed that Deccani, Nellore and Sonadi breeds fall under one clade, whereas Chokla, Malpura and Nali breeds fall under a separate clade. The results indicated that these breeds might have diverged many generations ago and estimated to be from 219333 to 440333 years ago. These results suggest that the mt-DNAD-loop analysis can serve as a tool for understanding the genetic architecture and phylogenetic relationship of Indian sheep breeds.
ISSN:0971-9857
0973-9718
DOI:10.5958/0973-9718.2015.00039.2