Isolation, Screening, Characterization and Maintenance of Amylase Producing Bacteria from Soil

• Published in: International journal for research in applied science and engineering technology Vol. 10; no. 8; pp. 45 - 49
• Main Authors: Gadhave, Avinash, Wagh, Mahesh S, Kale, Pravin, Bhakare, Sagar
• Format: Journal Article
• Language: English
• Published: 31.08.2022
• ISSN: 2321-9653; 2321-9653
• DOI: 10.22214/ijraset.2022.46121

Amylases are amongst most widely used enzymes in industries such as food, fermentation, starch processing, textile, and paper. In the present investigation, bacteria were isolated from soil samples of different areas of Padmashri Vikhe Patil College, Pravaranagar, Maharashtra, India screened for the production of amylase. Soil samples were serially diluted in normal saline and plated on sterile nutrient agar plates. The colonies obtained from higher dilutions were subjected to Gram staining, biochemical reactions, and starch hydrolysis test. Bacterial colony yielding positive starch hydrolysis test were subjected to Amylase activity test by DNSA method. Characteristic feature of the strain indicates that it belongs to the genus Bacillus and will be later used for further characterization. Maximum yield of amylase was obtained after 48hrs of incubation. The isolated amylase producing organism was maintain by using agar slant & glycerol stock method.