Validation of the SafTest Peroxide Test Kit for the Measurement of the Peroxide Content of Oils, Tallows, Meat Meals, Potato Chips, and Grain-Based Snacks: AOAC Performance Tested Method SM 101001

• Published in: Journal of AOAC International Vol. 104; no. 2; pp. 325 - 338
• Main Authors: Gordon, Virginia, Rainey, Christopher, Studmire, Wilainia
• Format: Journal Article
• Language: English
• Published: 21.05.2021
• ISSN: 1060-3271; 1944-7922
• DOI: 10.1093/jaoacint/qsaa155

Abstract Background Peroxide value is used as an indicator of oxidative degradation, off flavor, and rancidity in fat. Traditional methods are dependent on a fat extraction step to extract fat for subsequent titration of peroxides. The SafTest Peroxide Test offers a direct measurement of the peroxide value of vegetable oils, animal fats, meat meal and fish meal products, and potato chips, crackers, and other processed grain-based snack products using micro-analytical and membrane separation principles. Objective The study objective is to validate the SafTest Peroxide Test in an internal study, two contracted studies, and an independent laboratory study. Method Calibration, LOQ, robustness, selectivity, matrix, shelf life, and lot-to-lot stability were evaluated. Results The practical dynamic range of the calibration is 0.00 to 0.20 meq/kg. LOQ was determined as 0.02 meq/kg for liquids analyzed neat and 0.08 to 0.46 meq/kg for dry matrixes requiring a 1:4 to a 1:8 dilution. SafTest Peroxide Test relative standard deviations of repeatability (RSDr) averaged 1–13% compared to AOCS Cd 8–53 RSDr% of 1–15% and demonstrated average recoveries of 95.7, 91.5, and 105.2% of the AOCS Cd 8–53 results for olive oil, meat meal and fish, and animal fat and oils, respectively. Conclusions The SafTest Peroxide Test is an acceptable alternative to AOCS Cd 8–53 and AOCS Cd 8 b-90 for the measurement of peroxide value in oils, fats, meat meal, fish meal, and snack products. Highlights The SafTest Peroxide Test uses small reagent volumes, eliminates fat extraction, and provides rapid and standardized procedures for the detection of peroxide.