CDC BOTTLE BIOASSAYS FOR DETECTING INSECTICIDE RESISTANCE IN AEDES ALBOPICTUS, AEDES KOREICUS, AND CULEX PIPIENS FROM U.S. ARMY GARRISONS IN THE REPUBLIC OF KOREA (2023 AND 2024)

• Published in: Journal of the American Mosquito Control Association
• Main Authors: Stoops, Craig A, Kim, Myong Sun, Chong, Sung Tae, Cinkovich, Stephanie S, Carder, Jennifer B
• Format: Journal Article
• Language: English
• Published: United States 08.07.2025
• Subjects: Culex pipiens; Aedes albopictus; CDC Bottle Bioassay; Republic of Korea; Aedes koreicus
• ISSN: 1943-6270
• DOI: 10.2987/25-7225

Mosquito-borne pathogens are a threat to US troops stationed in the Republic of Korea. From April 2023 until December 2024, larvae and pupae of Aedes albopictus, Ae. koreicus, and Culex pipiens collected from U.S. Army garrisons were screened for resistance to chlorpyrifos, deltamethrin, etofenprox and permethrin using the Centers for Disease Control and Prevention bottle bioassay. Colonies of Cx. pipens var. molestus from Camp Humphreys, Camp George, and the Busan Storage Facility were established and tested against permethrin and permethrin + pieronyl butoxide (PBO). No resistance to deltamethrin or chlorpyrifos was detected in Ae. albopictus populations, but 1 population from Camp George showed possible resistance to permethrin. Aedes koreicus indicated possible resistance to etofenprox from Camp Casey in 2023 and resistance to etofenprox from Camp Casey in 2024. All populations of Ae. koreicus tested were susceptible to chlorpyifos, permethrin, and deltamethrin. Culex pipiens field-collected populations were susceptible to chlorpyrifos and resistant to permethrin, etofenprox, and deltamethrin. Exposing laboratory Cx. pipiens var. molestus to PBO and then permethrin restored susceptibility to all 3 colonies. Screening will be ongoing to track changes in resistance status for these species.